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NCKAP1 antibody [N1N2], N-term

Orthogonal
Anti-NCKAP1 antibody [N1N2], N-term used in IHC (Frozen sections) (IHC-Fr). GTX115481
Anti-NCKAP1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX115481
Anti-NCKAP1 antibody [N1N2], N-term used in Western Blot (WB). GTX115481
Anti-NCKAP1 antibody [N1N2], N-term used in Western Blot (WB). GTX115481
Anti-NCKAP1 antibody [N1N2], N-term used in Western Blot (WB). GTX115481

Cat. No. GTX115481

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NCKAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

129 kDa. ( Note )

Predict Reactivity

Zebrafish, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.36 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human NCKAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11165778

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NCK associated protein 1 , HEM2 , NAP1 , NAP125 , p125Nap1

Cellular Localization

Cell membrane; Single-pass membrane protein; Cytoplasmic side , Cell projection , lamellipodium membrane; Single-pass membrane protein; Cytoplasmic side

Background

Is part of lamellipodial complex that controls Rac-dependent actin remodeling.

Database

Research Area

DATA IMAGES

Anti-NCKAP1 antibody [N1N2], N-term used in IHC (Frozen sections) (IHC-Fr). GTX115481

GTX115481 IHC-Fr Image

NCKAP1 antibody [N1N2], N-term detects NCKAP1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: NCKAP1 stained by NCKAP1 antibody [N1N2], N-term (GTX115481) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NCKAP1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX115481

GTX115481 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung Papillary adenocarcinoma, using NCKAP1(GTX115481) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NCKAP1 antibody [N1N2], N-term used in Western Blot (WB). GTX115481

GTX115481 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NCKAP1 antibody [N1N2], N-term (GTX115481) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NCKAP1 antibody [N1N2], N-term used in Western Blot (WB). GTX115481

GTX115481 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NCKAP1 antibody [N1N2], N-term (GTX115481) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NCKAP1 antibody [N1N2], N-term used in Western Blot (WB). GTX115481

GTX115481 WB Image

Orthogonal

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NCKAP1 antibody [N1N2], N-term (GTX115481) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169