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NCOR1 antibody

Orthogonal
Anti-NCOR1 antibody used in Western Blot (WB). GTX129621
Anti-NCOR1 antibody used in Immunoprecipitation (IP). GTX129621

Cat. No. GTX129621

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all NCOR1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

270 kDa. ( Note )

Positive Control

HeLa nuclear extract

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NCOR1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886057

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear receptor corepressor 1 , N-CoR , N-CoR1 , PPP1R109 , TRAC1 , hN-CoR

Cellular Localization

Nucleus

Background

This gene encodes a protein that mediates ligand-independent transcription repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. It is part of a complex which also includes histone deacetylases and transcriptional regulators similar to the yeast protein Sin3p. This gene is located between the Charcot-Marie-Tooth and Smith-Magenis syndrome critical regions on chromosome 17. An alternatively spliced transcript variant has been described, but its full length sequence has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NCOR1 antibody used in Western Blot (WB). GTX129621

GTX129621 WB Image

Orthogonal

NCOR1 antibody detects NCOR1 protein by western blot analysis.
A. 30 μg HeLa whole cell extract
B. 30 μg HeLa nuclear extract
5 % SDS-PAGE
NCOR1 antibody (GTX129621) dilution: 1:500

Anti-NCOR1 antibody used in Immunoprecipitation (IP). GTX129621

GTX129621 IP Image

Immunoprecipitation of NCOR1 protein from HeLa whole cell extracts using 5 μg of NCOR1 antibody (GTX129621).
Western blot analysis was performed using NCOR1 antibody (GTX129621) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169