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NCX1 antibody [C2C12]

Anti-NCX1 antibody [C2C12] used in IHC (Paraffin sections) (IHC-P). GTX22869
Anti-NCX1 antibody [C2C12] used in IHC (Paraffin sections) (IHC-P). GTX22869
Anti-NCX1 antibody [C2C12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22869
Anti-NCX1 antibody [C2C12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22869

Cat. No. GTX22869

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 C2C12

Isotype

 IgM

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP

Reactivity

 Human, Mouse, Rat, Rabbit, Bovine, Dog, Guinea pig, Pig, Marsupials, Rodent
Package
100 μl ($399)
See all NCX1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1,000
ICC/IF 1:200
IHC-P 1:100
IHC-Fr Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

108 kDa. ( Note )

Product Note

This antibody recognizes an epitope between amino acids 371-525, which is on the intracellular side of the plasma membrane.

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Dog

Immunogen

Purified canine cardiac sodium/calcium exchanger.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 8 member A1 , NCX1

Cellular Localization

Cell membrane

Database

Research Area

DATA IMAGES

Anti-NCX1 antibody [C2C12] used in IHC (Paraffin sections) (IHC-P). GTX22869

GTX22869 IHC-P Image

IHC-P analysis of human heart tissue using GTX22869 NCX1 antibody [C2C12].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

Anti-NCX1 antibody [C2C12] used in IHC (Paraffin sections) (IHC-P). GTX22869

GTX22869 IHC-P Image

IHC-P analysis of human kidney tissue using GTX22869 NCX1 antibody [C2C12].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:50

Anti-NCX1 antibody [C2C12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22869

GTX22869 ICC/IF Image

ICC/IF analysis of A2058 Cells using GTX22869 NCX1 antibody [C2C12]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

Anti-NCX1 antibody [C2C12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22869

GTX22869 ICC/IF Image

ICC/IF analysis of U251 cells using GTX22869 NCX1 antibody [C2C12]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

REFERENCE

Application Reference

Knudsen JR et al. Mol Metab 2020; 39 : 100998 Prior exercise in humans redistributes intramuscular GLUT4 and enhances insulin-stimulated sarcolemmal and endosomal GLUT4 translocation.
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399