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NDRG1 antibody [AT11G4]

Anti-NDRG1 antibody [AT11G4] used in Western Blot (WB). GTX57654
Anti-NDRG1 antibody [AT11G4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57654

Cat. No. GTX57654

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT11G4

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all NDRG1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT11G4 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human NDRG3 protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

N-myc downstream regulated 1 , CAP43 , CMT4D , DRG1 , GC4 , HMSNL , NDR1 , NDRG1 , NMSL , Nmyc downstream regulated 1 , PROXY1 , RIT42 , RTP , TARG1 , TDD5

Cellular Localization

Cytoplasm,Cytosol,Cytoskeleton,Microtubule organizing center,Centrosome,Nucleus,Cell membrane

Background

This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

Database

Research Area

DATA IMAGES

Anti-NDRG1 antibody [AT11G4] used in Western Blot (WB). GTX57654

GTX57654 WB Image

WB analysis of various samples using GTX57654 NDRG1 antibody.
Lane 1 : LNCap whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : PC-3 whole cell lysate
Lane 4 : Hep3B whole cell lysate
Lane 5 : HT1080 whole cell lysate
Lane 6 : Jurkat whole cell lysate
Lane 7 : SW480 whole cell lysate
Loading : 40 μg
Dilution : 1:1000

Anti-NDRG1 antibody [AT11G4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57654

GTX57654 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX57654 NDRG1 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399