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NDUFA5 antibody [N1C3]

Orthogonal
Anti-NDUFA5 antibody [N1C3] used in Western Blot (WB). GTX111016
Anti-NDUFA5 antibody [N1C3] used in Western Blot (WB). GTX111016
Anti-NDUFA5 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX111016
Anti-NDUFA5 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX111016
Anti-NDUFA5 antibody [N1C3] used in Western Blot (WB). GTX111016

Cat. No. GTX111016

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all NDUFA5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Positive Control

Mouse brain , HepG2

Predict Reactivity

Rat, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.47 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human NDUFA5 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037494

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NADH:ubiquinone oxidoreductase subunit A5 , B13 , CI-13KD-B , CI-13kB , NUFM , UQOR13

Cellular Localization

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side

Background

The human NDUFA5 gene codes for the B13 subunit of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. The high degree of conservation of NDUFA5 extending to plants and fungi indicates its functional significance in the enzyme complex. The protein localizes to the inner mitochondrial membrane as part of the 7 component-containing, water soluble "iron-sulfur protein" (IP) fraction of complex I, although its specific role is unknown. It is assumed to undergo post-translational removal of the initiator methionine and N-acetylation of the next amino acid. The predicted secondary structure is primarily alpha helix, but the carboxy-terminal half of the protein has high potential to adopt a coiled-coil form. The amino-terminal part contains a putative beta sheet rich in hydrophobic amino acids that may serve as mitochondrial import signal. Related pseudogenes have also been identified on four other chromosomes. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NDUFA5 antibody [N1C3] used in Western Blot (WB). GTX111016

GTX111016 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with NDUFA5 antibody [N1C3] (GTX111016) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-NDUFA5 antibody [N1C3] used in Western Blot (WB). GTX111016

GTX111016 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with NDUFA5 antibody [N1C3] (GTX111016) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NDUFA5 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX111016

GTX111016 IHC-P Image

NDUFA5 antibody [N1C3] detects NDUFA5 protein at mitochondria in human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
NDUFA5 antibody [N1C3] (GTX111016) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NDUFA5 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX111016

GTX111016 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NDUFA5(GTX111016) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NDUFA5 antibody [N1C3] used in Western Blot (WB). GTX111016

GTX111016 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
15% SDS PAGE
GTX111016 diluted at 1:1000

REFERENCE

Application Reference

Lee H et al. Nat Commun 2023; 14 (1) : 6721 The mitochondrial fusion protein OPA1 is dispensable in the liver and its absence induces mitohormesis to protect liver from drug-induced injury.
Lee H et al. J Biol Chem 2020; 295 (19) : 6543-6560 The short variant of optic atrophy 1 (OPA1) improves cell survival under oxidative stress.
Lee H et al. J Biol Chem 2017; (Epub) The Short Variant of the Mitochondrial Dynamin OPA1 Maintains Mitochondrial Energetics and Cristae Structure.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169