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NDUFB10 antibody [N1C3]

Anti-NDUFB10 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114576
Anti-NDUFB10 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX114576
Anti-NDUFB10 antibody [N1C3] used in Western Blot (WB). GTX114576
Anti-NDUFB10 antibody [N1C3] used in Western Blot (WB). GTX114576

Cat. No. GTX114576

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NDUFB10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619231

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NADH:ubiquinone oxidoreductase subunit B10 , PDSW

Cellular Localization

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side

Database

Research Area

DATA IMAGES

Anti-NDUFB10 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114576

GTX114576 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using NDUFB10(GTX114576) antibody at 1:200 dilution.

Anti-NDUFB10 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX114576

GTX114576 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using NDUFB10(GTX114576) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NDUFB10 antibody [N1C3] used in Western Blot (WB). GTX114576

GTX114576 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with NDUFB10 antibody [N1C3] (GTX114576) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-NDUFB10 antibody [N1C3] used in Western Blot (WB). GTX114576

GTX114576 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with NDUFB10 antibody [N1C3] (GTX114576) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

There are currently no references for NDUFB10 antibody [N1C3] (GTX114576). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169