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NEK2 antibody

Anti-NEK2 antibody used in Western Blot (WB). GTX54614
Anti-NEK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX54614
Anti-NEK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX54614

Cat. No. GTX54614

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all NEK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human NEK2 (NP_001191112.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NIMA related kinase 2 , HsPK21 , NEK2A , NLK1 , PPP1R111 , RP67

Cellular Localization

Cytoplasm,Nucleolus

Background

This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene. [provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES

Anti-NEK2 antibody used in Western Blot (WB). GTX54614

GTX54614 WB Image

WB analysis of various sample lysates using GTX54614 NEK2 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-NEK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX54614

GTX54614 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX54614 NEK2 antibody.
Dilution : 1:100

Anti-NEK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX54614

GTX54614 IHC-P Image

IHC-P analysis of human esophagus tissue using GTX54614 NEK2 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399