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NF-L antibody [DA2]

Anti-NF-L antibody [DA2] used in ELISA (ELISA). GTX24572
Anti-NF-L antibody [DA2] used in ELISA (ELISA). GTX24572
Anti-NF-L antibody [DA2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24572
Anti-NF-L antibody [DA2] used in Western Blot (WB). GTX24572

Cat. No. GTX24572

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 DA2

Isotype

 IgG1

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse, Rat, Bovine, Pig
Package
100 μl ($399)
See all NF-L products

APPLICATION

Application Note

IF: Use at a dilution of 1/100 - 1/500. IHC-Fr: Use at an assay dependant dilution. WB: Use at a dilution of 1/5000 - 1/10000. Predicted molecular weight: 68 kDa. Optimal dilutions/concentrations should be determined by the end user.

Product Note

Screening was performed by ELISA using the immunogen, followed by immunofluorescence microscopy. Clones which revealed strong staining were further characterized biochemically.

PROPERTIES

Form

Liquid

Buffer

Tissue culture supernatant

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Pig

Immunogen

Enzymatically dephosphorylated pig neurofilaments including NF-L, NF-M and NF-H.

Purification

Tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Cytoplasmic

Background

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-L antibody can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease. Mutations in the protein coding region of the human NF-L gene appear to cause some forms of Charcot-Marie-Tooth disease.

Research Area

DATA IMAGES

Anti-NF-L antibody [DA2] used in ELISA (ELISA). GTX24572

GTX24572 ELISA Image

Sandwich ELISA detection of recombinant full-length NF-L protein using GTX101142 as capture antibody at concentration of 5 μg/mL and GTX24572 as detection antibody was diluted at 1:1000. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-NF-L antibody [DA2] used in ELISA (ELISA). GTX24572

GTX24572 ELISA Image

Sandwich ELISA detection of recombinant full-length NF-L protein using GTX134085 as capture antibody at concentration of 5 μg/mL and GTX24572 as detection antibody was diluted at 1:1000. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-NF-L antibody [DA2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24572

GTX24572 ICC/IF Image

Cells grown from adult rat brain. Large cell in middle is stained with mouse monoclonal to NF-L clone DA2 (green). Another type of neuronal lineage cell was stained with rabbit polyclonal to alpha-internexin RPCa-a-Int (red). These cells were mitotic but had several characteristics of neurons.

Anti-NF-L antibody [DA2] used in Western Blot (WB). GTX24572

GTX24572 WB Image

Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stainted with various antibodies including MCA-DA2 (lane 4).

REFERENCE

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Package List Price ($)
$ 399