GeneTex

  • Country / Location Selection

United States (US)

NF-M antibody [GT883]

Anti-NF-M antibody [GT883] used in IHC (Frozen sections) (IHC-Fr). GTX634483
Anti-NF-M antibody [GT883] used in IHC (Paraffin sections) (IHC-P). GTX634483
Anti-NF-M antibody [GT883] used in Western Blot (WB). GTX634483
Anti-NF-M antibody [GT883] used in IHC (Paraffin sections) (IHC-P). GTX634483
Anti-NF-M antibody [GT883] used in IHC (Paraffin sections) (IHC-P). GTX634483
Anti-NF-M antibody [GT883] used in IHC (Paraffin sections) (IHC-P). GTX634483
Anti-NF-M antibody [GT883] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634483
Anti-NF-M antibody [GT883] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634483

Cat. No. GTX634483

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT883

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IHC

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NF-M products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:25000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

96 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.25 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse NF-M. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888460

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neurofilament, medium polypeptide , NF-M , NF160 , NF165 , Nef3 , Nfm

Database

Research Area

DATA IMAGES

Anti-NF-M antibody [GT883] used in IHC (Frozen sections) (IHC-Fr). GTX634483

GTX634483 IHC-Fr Image

NF-M antibody [GT883] detects NF-M protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse mouse cerebellum.
Green: NF-M stained by NF-M antibody [GT883] (GTX634483) diluted at 1:250.
Red: Calbindin, stained by Calbindin antibody (GTX130856) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NF-M antibody [GT883] used in IHC (Paraffin sections) (IHC-P). GTX634483

GTX634483 IHC-P Image

NF-M antibody [GT883] detects NF-M protein at by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
NF-M stained by NF-M antibody [GT883] (GTX634483) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NF-M antibody [GT883] used in Western Blot (WB). GTX634483

GTX634483 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NF-M antibody [GT883] (GTX634483) diluted at 1:25000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-NF-M antibody [GT883] used in IHC (Paraffin sections) (IHC-P). GTX634483

GTX634483 IHC-P Image

NF-M antibody [GT883] detects NF-M protein at by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
NF-M stained by NF-M antibody [GT883] (GTX634483) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NF-M antibody [GT883] used in IHC (Paraffin sections) (IHC-P). GTX634483

GTX634483 IHC-P Image

NF-M antibody [GT883] detects NF-M protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
NF-M stained by NF-M antibody [GT883] (GTX634483) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NF-M antibody [GT883] used in IHC (Paraffin sections) (IHC-P). GTX634483

GTX634483 IHC-P Image

NF-M antibody [GT883] detects NF-M protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
NF-M stained by NF-M antibody [GT883] (GTX634483) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NF-M antibody [GT883] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634483

GTX634483 ICC/IF Image

NF-M antibody [GT883] detects NF-M protein at axon by immunofluorescent analysis.
Sample: DIV10 rat E18 primary hippocampal neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NF-M stained by NF-M antibody [GT883] (GTX634483) diluted at 1:250.
Red: Tau, a cytoskeleton marker, stained by Tau antibody (GTX130462) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NF-M antibody [GT883] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634483

GTX634483 ICC/IF Image

The data was published in the 2019 in Sci Rep. PMID: 30931957

REFERENCE

Application Reference

Orikasa S et al. Sci Rep 2022; 12 (1) : 682 Hypoxia-inducible factor 1α induces osteo/odontoblast differentiation of human dental pulp stem cells via Wnt/β-catenin transcriptional cofactor BCL9.
Application : ICC/IF
Reactivity : Human
Noda S et al. Sci Rep 2019; 9 (1) : 5430 Effect of cell culture density on dental pulp-derived mesenchymal stem cells with reference to osteogenic differentiation.
Application : IHC
Reactivity : Mouse
Submit a Reference

REVIEW

There are currently no reviews for NF-M antibody [GT883] (GTX634483). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations