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NF-M antibody

Orthogonal
Anti-NF-M antibody used in IHC (Frozen sections) (IHC-Fr). GTX133215
Anti-NF-M antibody used in IHC (Paraffin sections) (IHC-P). GTX133215
Anti-NF-M antibody used in Western Blot (WB). GTX133215
Anti-NF-M antibody used in IHC (Paraffin sections) (IHC-P). GTX133215
Anti-NF-M antibody used in Western Blot (WB). GTX133215

Cat. No. GTX133215

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NF-M products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:10000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

96 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Human, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse NF-M. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886873

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neurofilament, medium polypeptide , NF-M , NF160 , NF165 , Nef3 , Nfm

Background

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.

Database

Research Area

DATA IMAGES

Anti-NF-M antibody used in IHC (Frozen sections) (IHC-Fr). GTX133215

GTX133215 IHC-Fr Image

NF-M antibody detects NF-M protein expression by immunohistochemical analysis.
Sample:Frozen-sectioned adult mouse cerebellum.
Green: NF-M protein stained by NF-M antibody (GTX133215) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-NF-M antibody used in IHC (Paraffin sections) (IHC-P). GTX133215

GTX133215 IHC-P Image

NF-M antibody detects NF-M protein at cell membrane and cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
NF-M antibody (GTX133215) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NF-M antibody used in Western Blot (WB). GTX133215

GTX133215 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NF-M antibody (GTX133215) diluted at 1:10000.

Anti-NF-M antibody used in IHC (Paraffin sections) (IHC-P). GTX133215

GTX133215 IHC-P Image

NF-M antibody detects NF-M protein at cell membrane and cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
NF-M antibody (GTX133215) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NF-M antibody used in Western Blot (WB). GTX133215

GTX133215 WB Image

Orthogonal

The data was published in the journal Acta Neuropathol Commun in 2019. PMID: 30626447

REFERENCE

Application Reference

Gama Sosa MA et al. Acta Neuropathol Commun 2019; 7 (1) : 6 Low-level blast exposure disrupts gliovascular and neurovascular connections and induces a chronic vascular pathology in rat brain.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169