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NFAT1 antibody [25A10.D6.D2]

Orthogonal
Anti-NFAT1 antibody [25A10.D6.D2] used in IHC (Paraffin sections) (IHC-P). GTX22722
Anti-NFAT1 antibody [25A10.D6.D2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22722
Anti-NFAT1 antibody [25A10.D6.D2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22722
Anti-NFAT1 antibody [25A10.D6.D2] used in ChIP assay (ChIP assay). GTX22722
Anti-NFAT1 antibody [25A10.D6.D2] used in IHC (Paraffin sections) (IHC-P). GTX22722
Anti-NFAT1 antibody [25A10.D6.D2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22722
Anti-NFAT1 antibody [25A10.D6.D2] used in IHC (Paraffin sections) (IHC-P). GTX22722
Anti-NFAT1 antibody [25A10.D6.D2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22722

Cat. No. GTX22722

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 25A10.D6.D2

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ChIP assay, Gel supershift assays

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all NFAT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/ml
ICC/IF 1:100 - 1:1000
IHC-P 1:50-1:200
IHC-Fr 1:50-1:200
FACS Assay dependent
IP Assay dependent
ChIP assay 1-3 μl
Gel supershift assays Assay dependent
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Product Note

This antibody does not cross react with NFAT2 (NFATc, NFATc1).

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide corresponding to residues A(51) I S S P S G L A Y P D D V L D Y G L(69) of mouse NFATc2-A, B and C isoforms.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_372490

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2 , AI607462 , NF-ATc2 , NF-ATp , NFAT1 , NFAT1-D , Nfatp

Cellular Localization

Cytoplasm,Nucleus

Background

The nuclear factor of activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that collectively regulate the immune response. Four NFAT proteins, encoded on separate genes and expressed as several splice variants, have been described: NFAT1 (also known as NFATp or NFATc2), NFAT2 (NFATc or NFATc1), NFAT3, and NFAT4 (NFATx or NFATc3). These proteins show a low level of sequence similarity with the Dorsal/Rel/NFkB family of transcription factors. Another NFAT-related protein termed NFAT5 differs from isoforms 1-4 in that it lacks many of the Fos/Jun contact sites observed in its predecessors and its subcellular localization is not calcineurin-dependent.

Database

Research Area

DATA IMAGES

Anti-NFAT1 antibody [25A10.D6.D2] used in IHC (Paraffin sections) (IHC-P). GTX22722

GTX22722 IHC-P Image

IHC-P analysis of human spleen tissue using GTX22722 NFAT1 antibody [25A10.D6.D2].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

Anti-NFAT1 antibody [25A10.D6.D2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22722

GTX22722 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22722 NFAT1 antibody [25A10.D6.D2]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

Anti-NFAT1 antibody [25A10.D6.D2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22722

GTX22722 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX22722 NFAT1 antibody [25A10.D6.D2]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

Anti-NFAT1 antibody [25A10.D6.D2] used in ChIP assay (ChIP assay). GTX22722

GTX22722 ChIP assay Image

Orthogonal

ChIP analysis of 1x10⁶ HTC-IR rat hepatoma cells treated with insulin for 0, 10, and 30 minutes using GTX22722 NFAT1 antibody [25A10.D6.D2]. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (refer to PMID: 22098709). The precipitated DNA was detected by PCR with primer set targeting to exon-1 or exon-28 of the LAMC1 gene.
IP reaction : 1.0ul antibody / 100ul sample

Anti-NFAT1 antibody [25A10.D6.D2] used in IHC (Paraffin sections) (IHC-P). GTX22722

GTX22722 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX22722 NFAT1 antibody [25A10.D6.D2].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

Anti-NFAT1 antibody [25A10.D6.D2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22722

GTX22722 ICC/IF Image

Orthogonal

ICC/IF analysis of HeLa cells untreated (left panel) or treated with 1uM staurosporine (right panel) for 3 hours using GTX22722 NFAT1 antibody [25A10.D6.D2].
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature

Anti-NFAT1 antibody [25A10.D6.D2] used in IHC (Paraffin sections) (IHC-P). GTX22722

GTX22722 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX22722 NFAT1 antibody [25A10.D6.D2].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

Anti-NFAT1 antibody [25A10.D6.D2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22722

GTX22722 ICC/IF Image

ICC/IF analysis of U251 cells using GTX22722 NFAT1 antibody [25A10.D6.D2]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

REFERENCE

Application Reference

Zhou Y et al. Nat Commun 2024; 15 (1) : 1405 Cell softness renders cytotoxic T lymphocytes and T leukemic cells resistant to perforin-mediated killing.
Garaud S et al. J Immunol 2011; 186 (8) : 4835-44 CD5 promotes IL-10 production in chronic lymphocytic leukemia B cells through STAT3 and NFAT2 activation.
Application : ChIP assay
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399