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NFkB p105/p50 antibody

Anti-NFkB p105/p50 antibody used in Western Blot (WB). GTX27549

Cat. No. GTX27549

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA, EMSA

Reactivity

 Human
Package
100 μl ($589)
See all NFkB p105/p50 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ELISA 1:5000-1:25000
EMSA 0.5-1 μL
Not tested in other applications.

Calculated MW

105 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filtered-steriled serum

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

80 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human NFkB p50 (NFKB1) peptide corresponding to a region near the N-terminus of the human protein conjugated to KLH.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_370045

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear factor kappa B subunit 1 , CVID12 , EBP-1 , KBF1 , NF-kB1 , NF-kappa-B1 , NF-kappaB , NFKB-p105 , NFKB-p50 , NFkappaB , p105 , p50

Cellular Localization

Nucleus,Cytoplasm

Background

The transcription factor NFkB is widely recognized as a critical mediator of immune and inflammatory responses. In most cell types, NFkB is found in the cytoplasm where it is associated with an inhibitory protein known as IkB. An impressive variety of stimuli (tumor necrosis factor, interleukin 1, T-cell activation signals, bacterial endotoxins, viral transforming proteins, certain growth factors and reactive oxygen intermediates) lead to the rapid nuclear accumulation of NFkB by the induced phosphorylation and subsequent degradation of IkB. In the nucleus, NFkB regulates genes encoding cytokines, cytokine receptors, cell adhesion molecules, proteins involved in coagulation and genes involved in cell growth control. Additionally, NFkB is thought to be an important transcriptional regulator for HIV. Growing evidence indicates that the disregulation of NFkB may be key to a number of diseases including arthritis and other inflammatory diseases, Alzheimer's disease, atherosclerosis and cancer.

Database

Research Area

DATA IMAGES

Anti-NFkB p105/p50 antibody used in Western Blot (WB). GTX27549

GTX27549 WB Image

Western blot of HeLa cell extract.  The membrane was blocked in 5% dry milk for 2 h.  After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h.  Washes with buffer were performed 4 times for 5' each.  The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h.   Washes with TBS preceded color development.

REFERENCE

Application Reference

Adrianto I et al. Nat Genet 2011; 43 (3) : 253-8 Association of a functional variant downstream of TNFAIP3 with systemic lupus erythematosus.
Application : EMSA
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 589