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NFkB p65 (phospho Ser276) antibody

Orthogonal
Anti-NFkB p65 (phospho Ser276) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54672
Anti-NFkB p65 (phospho Ser276) antibody used in IHC (Paraffin sections) (IHC-P). GTX54672
Anti-NFkB p65 (phospho Ser276) antibody used in IHC (Paraffin sections) (IHC-P). GTX54672
Anti-NFkB p65 (phospho Ser276) antibody used in IHC (Paraffin sections) (IHC-P). GTX54672
Anti-NFkB p65 (phospho Ser276) antibody used in Western Blot (WB). GTX54672

Cat. No. GTX54672

Reference 11

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all NFkB p65 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:20 - 1:50
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic phosphorylated peptide around S276 of human RELA (NP_068810.3).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RELA proto-oncogene, NF-kB subunit , CMCU , NFKB3 , p65

Cellular Localization

Nucleus,Cytoplasm

Background

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-NFkB p65 (phospho Ser276) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54672

GTX54672 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX54672 NFkB p65 (phospho Ser276) antibody.
Blue : DAPI
Dilution : 1:100

Anti-NFkB p65 (phospho Ser276) antibody used in IHC (Paraffin sections) (IHC-P). GTX54672

GTX54672 IHC-P Image

IHC-P analysis of rat liver tissue using GTX54672 NFkB p65 (phospho Ser276) antibody.
Dilution : 1:100

Anti-NFkB p65 (phospho Ser276) antibody used in IHC (Paraffin sections) (IHC-P). GTX54672

GTX54672 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX54672 NFkB p65 (phospho Ser276) antibody.
Dilution : 1:100

Anti-NFkB p65 (phospho Ser276) antibody used in IHC (Paraffin sections) (IHC-P). GTX54672

GTX54672 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX54672 NFkB p65 (phospho Ser276) antibody.
Dilution : 1:100

Anti-NFkB p65 (phospho Ser276) antibody used in Western Blot (WB). GTX54672

GTX54672 WB Image

Orthogonal

WB analysis of HeLa cells, NIH/3T3, and C6 cells treated by TNF-α (20 ng/ml) at 37ºC for 30 minutes and HeLa cells treated by Calyculin A (100 nM) at 37ºC for 30 minutes after serum-starvation overnight using GTX54672 NFkB p65 (phospho Ser276) antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

Application Reference

Ragab SMM et al. Sci Rep 2024; 14 (1) : 932 Protective effect of Moringa oleifera leaf ethanolic extract against uranyl acetate-induced testicular dysfunction in rats.
RM Yehia et al. Sci Rep 2024; 14 (1) : 1359 Resolving acne with optimized adapalene microspongeal gel, in vivo and clinical evaluations
Application : IHC-P
Morsy MA et al. Pharmaceuticals (Basel) 2023; 16 (5) Pregnenolone Inhibits Doxorubicin-Induced Cardiac Oxidative Stress, Inflammation, and Apoptosis-Role of Matrix Metalloproteinase 2 and NADPH Oxidase 1.
Salama RM et al. Arch Pharm Res 2023; 46 (4) : 323-338 Regulation of NOX/p38 MAPK/PPARα pathways and miR-155 expression by boswellic acids reduces hepatic injury in experimentally-induced alcoholic liver disease mouse model: novel mechanistic insight.
Chen MZ et al. Int J Mol Sci 2022; 23 (16) Home Dust Mites Promote MUC5AC Hyper-Expression by Modulating the sNASP/TRAF6 Axis in the Airway Epithelium.
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REVIEW

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Application Reference
Ragab SMM et al. Sci Rep 2024; 14 (1):932 Protective effect of Moringa oleifera leaf ethanolic extract against uranyl acetate-induced testicular dysfunction in rats.
RM Yehia et al. Sci Rep 2024; 14 (1):1359 Resolving acne with optimized adapalene microspongeal gel, in vivo and clinical evaluations
Application : IHC-P
Morsy MA et al. Pharmaceuticals (Basel) 2023; 16 (5) Pregnenolone Inhibits Doxorubicin-Induced Cardiac Oxidative Stress, Inflammation, and Apoptosis-Role of Matrix Metalloproteinase 2 and NADPH Oxidase 1.
Salama RM et al. Arch Pharm Res 2023; 46 (4):323-338 Regulation of NOX/p38 MAPK/PPARα pathways and miR-155 expression by boswellic acids reduces hepatic injury in experimentally-induced alcoholic liver disease mouse model: novel mechanistic insight.
Abbas H et al. Pharmaceutics 2022; 14 (10) Development and Evaluation of Novel Leflunomide SPION Bioemulsomes for the Intra-Articular Treatment of Arthritis.
Application : IHC-P
Reactivity : Mouse
Zewail M et al. Pharmaceuticals (Basel) 2022; 16 (1) Novel Siprulina platensis Bilosomes for Combating UVB Induced Skin Damage.
Application : IHC-P
Reactivity : Rat
Mohamed ME et al. Pharmaceuticals (Basel) 2022; 15 (10) Agathis robusta Bark Extract Protects from Renal Ischemia-Reperfusion Injury: Phytochemical, In Silico and In Vivo Studies.
Chen MZ et al. Int J Mol Sci 2022; 23 (16) Home Dust Mites Promote MUC5AC Hyper-Expression by Modulating the sNASP/TRAF6 Axis in the Airway Epithelium.
Abdelkader NF et al. Biomed Pharmacother 2022; 145:112395 Inosine mitigated diabetic peripheral neuropathy via modulating GLO1/AGEs/RAGE/NF-κB/Nrf2 and TGF-β/PKC/TRPV1 signaling pathways.
Application : IHC-P
Reactivity : Rat
Yonghong Liu et al. Front Cell Dev Biol 2020; 8:593653 PECAM1 Combines With CXCR4 to Trigger Inflammatory Cell Infiltration and Pulpitis Progression Through Activating the NF-κB Signaling Pathway.
Application : WB, IHC-P
Reactivity : Rat
Wang Y et al. Aging (Albany NY) 2020; 12 (20):20184-20197 Interleukin-6 knockout reverses macrophage differentiation imbalance and alleviates cardiac dysfunction in aging mice.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399