GeneTex
United States (US)

NGF-beta recombinant protein

Cat No. GTX65184

Application ELISA, Functional Assay, Apuri, Blocking
Reactivity Human
Species Human
APPLICATION

Application Note

The ED50 as determined using PC12 cell line is 1.2-2 ng/ml and using DRG cell line is 0.8-1.5 ng/ml.

Calculated MW

16.95 kDa. ( Note )
PROPERTIES

Form

Lyophilized powder

Buffer

Sterile filtered and then lyophilized in 30 mM Sodium Acetate, pH 5.5 containing 1% HAS.

Storage

Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers. Store at -20ºC.

Antigen Species

Human

Expression System

CHO cells

Purification


Purity was assessed by SDS-PAGE (≥98%) and by HPLC (≥98%).

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

beta-NGF, NGF, MGC161428., HSAN5, beta Polypeptide, betaNGF, MGC161426, NGFB, beta NGF

Background

Nerve growth factor-beta (NGF-beta) is a potent neurotrophic factor that supports the growth and survivability of nerve and/or glial cells. The active form of human NGF-beta is a dimer, formed by two identical 119 amino acid subunits, which is held together by strong hydrophobic interactions. Recombinant human BGF-b produced in CHO cells is a homodimer, glycosylated, polypeptide chain containing 2 identical 119 amino acids and having a molecular weight of 16.95 kDa. The protein is purified by proprietary chromatographic techniques.

Research Area

Package List Price ($)
$ 279