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NHERF2 antibody [N2C3]

Anti-NHERF2 antibody [N2C3] used in Western Blot (WB). GTX101466
Anti-NHERF2 antibody [N2C3] used in Western Blot (WB). GTX101466

Cat. No. GTX101466

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all NHERF2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

Mouse liver , NT2D1

Predict Reactivity

Rat, Rabbit, Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

5.91 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NHERF2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2037516

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SLC9A3 regulator 2 , E3KARP , NHE3RF2 , NHERF-2 , NHERF2 , OCTS2 , SIP-1 , SIP1 , TKA-1

Cellular Localization

Endomembrane system; Peripheral membrane protein , Nucleus

Background

NHE3 (SLC9A3; MIM 182307) is a sodium/hydrogen exchanger in the brush border membrane of the proximal tubule, small intestine, and colon that plays a major role in transepithelial sodium absorption. SLC9A3R2, as well as SLC9A3R1 (MIM 604990) and protein kinase A phosphorylation, may play a role in NHE3 regulation.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-NHERF2 antibody [N2C3] used in Western Blot (WB). GTX101466

GTX101466 WB Image

NHERF-2 antibody detects NHERF-2 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10 % SDS-PAGE, and blotted with NHERF-2 antibody (GTX101466) diluted by 1:1000

Anti-NHERF2 antibody [N2C3] used in Western Blot (WB). GTX101466

GTX101466 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
15% SDS PAGE
GTX101466 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169