GeneTex

  • Country / Location Selection

United States (US)

NKAP antibody

Orthogonal
Anti-NKAP antibody used in Western Blot (WB). GTX120929
Anti-NKAP antibody used in Western Blot (WB). GTX120929
Anti-NKAP antibody used in IHC (Paraffin sections) (IHC-P). GTX120929
Anti-NKAP antibody used in Western Blot (WB). GTX120929
Anti-NKAP antibody used in IHC (Paraffin sections) (IHC-P). GTX120929

Cat. No. GTX120929

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NKAP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

HeLa , HeLa nuclear

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NKAP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2861141

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NFKB activating protein

Cellular Localization

Nucleus

Background

NKAP is a transcriptional repressor that associates with the NOTCH (see MIM 190198) corepressor complex and is required for T-cell development (Pajerowski et al., 2009 [PubMed 19409814]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-NKAP antibody used in Western Blot (WB). GTX120929

GTX120929 WB Image

Orthogonal

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NKAP antibody (GTX120929) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NKAP antibody used in Western Blot (WB). GTX120929

GTX120929 WB Image

Orthogonal

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NKAP antibody (GTX120929) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NKAP antibody used in IHC (Paraffin sections) (IHC-P). GTX120929

GTX120929 IHC-P Image

NKAP antibody detects NKAP protein at nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
NKAP antibody (GTX120929) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NKAP antibody used in Western Blot (WB). GTX120929

GTX120929 WB Image

Orthogonal

NKAP antibody detects NKAP protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NKAP antibody (GTX120929) at a dilution of 1:1000.

Anti-NKAP antibody used in IHC (Paraffin sections) (IHC-P). GTX120929

GTX120929 IHC-P Image

NKAP antibody detects NKAP protein at nucleus on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
NKAP antibody (GTX120929) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Li L et al. Mol Cell 2020; 80 (3) : 525-540.e9 Characterization of Metabolic Patterns in Mouse Oocytes during Meiotic Maturation.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

There are currently no reviews for NKAP antibody (GTX120929). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169