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NMDAR2D antibody

Anti-NMDAR2D antibody used in IHC (Free Floating) (IHC (Free Floating)). GTX04109
Anti-NMDAR2D antibody used in Western Blot (WB). GTX04109

Cat. No. GTX04109

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC (Free Floating)

Reactivity

 Human, Mouse, Rat
Package
50 μl ($669)
See all NMDAR2D products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC (Free Floating) Assay dependent
Not tested in other applications.

Calculated MW

143 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 1% BSA

Preservative

0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Peptide CRTQNRTHRGESLHR, corresponding to amino acid residues 345-359 (Extracellular, N-terminus) of rat NMDAR2D (Accession Q62645).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamate ionotropic receptor NMDA type subunit 2D , GluN2D

Cellular Localization

Cell membrane

Background

N-methyl-D-aspartate receptor subunit; expressed in mid-brain structures with peak expression at day P7 [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-NMDAR2D antibody used in IHC (Free Floating) (IHC (Free Floating)). GTX04109

GTX04109 IHC (Free Floating) Image

IHC (Free Floating) analysis of rat brain tissue using GTX04109 NMDAR2D antibody. Cells positive for NR2D but negative for parvalbumin (thick arrow, shown in Panel A) and cells positive for both NR2D and parvalbumin (vertical arrow) are both present.
Panel A : NMDAR2D staining (green) appears in a discreet population of cells (thin arrows).
Panel B : Parvalbumin staining (red) and DAPI staining (blue)
Dilution : 1:200

Anti-NMDAR2D antibody used in Western Blot (WB). GTX04109

GTX04109 WB Image

WB analysis of various tissue lysates using GTX04109 NMDAR2D antibody (Lane 1 and 2) preincubated with blocking peptide (Lane 3 and 4).
Lane 1 and 3 : Mouse brain
Lane 2 and 4 : Rat brain
Dilution : 1:200

REFERENCE

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REVIEW

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Package List Price ($)
$ 669