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NMT1 antibody [AT2C8]

Anti-NMT1 antibody [AT2C8] used in Western Blot (WB). GTX57729
Anti-NMT1 antibody [AT2C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57729

Cat. No. GTX57729

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT2C8

Isotype

 IgG2a

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all NMT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human NMT1 (1-496aa) purified from E. coli

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

N-myristoyltransferase 1 , NMT , NMT1 , Nmyristoyltransferase 1

Cellular Localization

Cytoplasm,Cytosol,Membrane

Background

Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase (NMT; EC 2.3.1.97) catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO (GNAO1; MIM 139311) (Duronio et al., 1992 [PubMed 1570339]).[supplied by OMIM, Nov 2008]

Database

Research Area

DATA IMAGES

Anti-NMT1 antibody [AT2C8] used in Western Blot (WB). GTX57729

GTX57729 WB Image

WB analysis of various samples using GTX57729 NMT1 antibody [AT2C8].
Lane 1 : HepG2 whole cell lysate
Lane 2 : 293T whole cell lysate
Loading : 40μg

Anti-NMT1 antibody [AT2C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57729

GTX57729 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX57729 NMT1 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399