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NOD2 antibody [2D9]

Anti-NOD2 antibody [2D9] used in Flow cytometry (FACS). GTX30615
Anti-NOD2 antibody [2D9] used in Flow cytometry (FACS). GTX30615
Anti-NOD2 antibody [2D9] used in Western Blot (WB). GTX30615
Anti-NOD2 antibody [2D9] used in Western Blot (WB). GTX30615
Anti-NOD2 antibody [2D9] used in Western Blot (WB). GTX30615
Anti-NOD2 antibody [2D9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30615
Anti-NOD2 antibody [2D9] used in Western Blot (WB). GTX30615

Cat. No. GTX30615

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2D9

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, IP

Reactivity

 Human, Mouse, Primate
Package
100 μl ($469)
See all NOD2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100 - 1:2000
ICC/IF 1:50 - 1:500
IHC-P 1:10 - 1:500
FACS Assay dependent
IP 1:10 - 1:500
Not tested in other applications.

Calculated MW

115 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human NOD2 protein corresponding to residues 28-301. [UniProt# Q9HC29]

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nucleotide binding oligomerization domain containing 2 , ACUG , BLAU , BLAUS , CARD15 , CD , CLR16.3 , IBD1 , NLRC2 , NOD2B , PSORAS1 , YAOS

Cellular Localization

Cytoplasm,Membrane,Mitochondrion

Background

This gene is a member of the Nod1/Apaf-1 family and encodes a protein with two caspase recruitment (CARD) domains and six leucine-rich repeats (LRRs). The protein is primarily expressed in the peripheral blood leukocytes. It plays a role in the immune response to intracellular bacterial lipopolysaccharides (LPS) by recognizing the muramyl dipeptide (MDP) derived from them and activating the NFKB protein. Mutations in this gene have been associated with Crohn disease and Blau syndrome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NOD2 antibody [2D9] used in Flow cytometry (FACS). GTX30615

GTX30615 FACS Image

FACS (Intracellular staining) analysis of Raji cells using GTX30615 NOD2 antibody [2D9].
Blue : Primary antibody
Orange : isotype control
Dilution : 5 μg/ml
Fixation : 4% PFA
Permibilization : 0.1% saponin

Anti-NOD2 antibody [2D9] used in Flow cytometry (FACS). GTX30615

GTX30615 FACS Image

FACS (Intracellular staining) analysis of THP-1 cells using GTX30615 NOD2 antibody [2D9].
Blue : Primary antibody
Orange : isotype control
Dilution : 5 μg/ml
Fixation : 4% PFA
Permibilization : 0.1% saponin

Anti-NOD2 antibody [2D9] used in Western Blot (WB). GTX30615

GTX30615 WB Image

WB analysis of THP-1, RAW264.7, and U2OS cell lysate using GTX30615 NOD2 antibody [2D9].
Dilution : 2 μg/ml

Anti-NOD2 antibody [2D9] used in Western Blot (WB). GTX30615

GTX30615 WB Image

WB analysis of THP-1 cell lysate using GTX30615 NOD2 antibody [2D9].
Dilution : 2 μg/ml

Anti-NOD2 antibody [2D9] used in Western Blot (WB). GTX30615

GTX30615 WB Image

WB analysis of HT-29 cell lysate using GTX30615 NOD2 antibody [2D9].
Loading : 50μg

Anti-NOD2 antibody [2D9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30615

GTX30615 ICC/IF Image

ICC/IF analysis of A431 cells using GTX30615 NOD2 antibody [2D9].
Green : primary antibody
Red : Actin
Blue : DAPI
Dilution : 1:200
Fixation : 10% formalin
Permibilization : 0.5% Triton-X100

Anti-NOD2 antibody [2D9] used in Western Blot (WB). GTX30615

GTX30615 WB Image

WB analysis of HEK293 overexpressing NOD2 cell lysate using GTX30615 NOD2 antibody [2D9].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 469