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NOL3 antibody

Anti-NOL3 antibody used in IHC (Paraffin sections) (IHC-P). GTX111883
Anti-NOL3 antibody used in Western Blot (WB). GTX111883
Anti-NOL3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111883

Cat. No. GTX111883

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all NOL3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

H1299 , HepG2

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.64 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NOL3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036255

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nucleolar protein 3 , ARC , FCM , MYP , NOP , NOP30

Cellular Localization

Isoform 1: Nucleus , nucleolus , Isoform 2: Cytoplasm

Background

This gene encodes an anti-apoptotic protein that has been shown to down-regulate the enzyme activities of caspase 2, caspase 8 and tumor protein p53. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NOL3 antibody used in IHC (Paraffin sections) (IHC-P). GTX111883

GTX111883 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using NOL3(GTX111883) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NOL3 antibody used in Western Blot (WB). GTX111883

GTX111883 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: Hep G2 (GTX27900)
12% SDS PAGE
GTX111883 diluted at 1:1000

Anti-NOL3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111883

GTX111883 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NOL3(GTX111883) antibody at 1:200 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169