APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1 - 2 μg/mL |
5 μg/mL |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
SW480 Cell Lysate(GTX23957)
Product Note
At least four isoforms are known to exist; this antibody will detect all four isoforms.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
NOSTRIN antibody was raised against a 17 amino acid peptide near the carboxy terminus of human NOSTRIN.The immunogen is located within amino acids 430 - 480 of NOSTRIN.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
nitric oxide synthase trafficking , DaIP2
Cellular Localization
Cytoplasmic vesicle,Cytoplasm, cytoskeleton
Background
Nitric oxide (NO) is a potent mediator in biologic processes such as neurotransmission, inflammatory response, and vascular homeostasis. NOSTRIN binds the enzyme responsible for NO production, endothelial NO synthase (ENOS; MIM 163729), and triggers the translocation of ENOS from the plasma membrane to vesicle-like subcellular structures, thereby attenuating ENOS-dependent NO production.[supplied by OMIM, Apr 2004]
Database
Research Area
DATA IMAGES
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GTX32144 IHC-P Image
IHC-P analysis of mouse kidney tissue using GTX32144 NOSTRIN antibody. Working concentration : 5 μg/ml
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GTX32144 WB Image
WB analysis of SW480 cell lysate using GTX32144 NOSTRIN antibody. Dilution : 1μg/ml (Lane A) and 2 μg/ml (Lane B)
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GTX32144 IHC-P Image
IHC-P analysis of mouse kidney tissue using GTX32144 NOSTRIN antibody. Working concentration : 20 μg/ml
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REFERENCE
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REVIEW
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