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NOTCH1 antibody

Anti-NOTCH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX28387
Anti-NOTCH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX28387

Cat. No. GTX28387

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human
Package
200 μl ($619)
See all NOTCH1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000-1:10000
IHC-P 1:1000-5000
ELISA 1:20000-1:100000
Not tested in other applications.

Calculated MW

273 kDa. ( Note )

Product Note

Notch1 antibody is directed against human NOTCH 1. No reaction is detected against NOTCH 2. No reactivity was observed against Mouse Notch. Other species have not been tested.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to an internal region near amino acid 2480-2510 of human Notch 1.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

notch receptor 1 , AOS5 , AOVD1 , TAN1 , hN1

Cellular Localization

Cell membrane

Background

Notch-1 is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.

Database

Research Area

DATA IMAGES

Anti-NOTCH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX28387

GTX28387 IHC-P Image

IHC-P analysis of human brain cerebellum tissue using Notch 1 antibody at a dilution of 1:500. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

Anti-NOTCH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX28387

GTX28387 IHC-P Image

IHC-P analysis of human cerebellum tissue using GTX28387 NOTCH1 antibody.
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 619