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NOTCH2 antibody

Anti-NOTCH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX28927
Anti-NOTCH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX28927
Anti-NOTCH2 antibody used in Western Blot (WB). GTX28927

Cat. No. GTX28927

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA, Multiplexing

Reactivity

 Human
Package
100 μl ($399)
See all NOTCH2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:400-1:2000
ICC/IF Assay dependent
IHC-P 1:200-1:800
ELISA 1:30000-1:90000
Multiplexing Assay dependent
Not tested in other applications.

Calculated MW

265 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to an internal region near amino acid residues 2390-2415 of human Notch 2 (the total protein is 2471 aa).

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

notch receptor 2 , AGS2 , HJCYS , hN2

Cellular Localization

Cytoplasm

Background

Synthesized in the endoplasmic reticulum as an inactive form, which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.

Database

Research Area

DATA IMAGES

Anti-NOTCH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX28927

GTX28927 IHC-P Image

IHC-P analysis of human kidney tissue using Notch 2 antibody at a dilution of 1:500. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

Anti-NOTCH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX28927

GTX28927 IHC-P Image

IHC-P analysis of human kidney tissue using GTX28927 NOTCH2 antibody.
Dilution : 1:500

Anti-NOTCH2 antibody used in Western Blot (WB). GTX28927

GTX28927 WB Image

WB analysis of Non-transfected (lane 4-6) and transfected (lane 1-3) SV40 whole cell lysate using GTX28927 NOTCH2 antibody.
Lane 1 and 3 : Lysate 24 hr post transfection
Lane 2 and 5 : Lysate 48 hr post transfection
Lane 3 and 6 : Lysate 72 hr post transfection
Loading : 100 μg
Dilution : 1:400

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399