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NPR-A antibody

Orthogonal
Anti-NPR-A antibody used in Western Blot (WB). GTX109810
Anti-NPR-A antibody used in IHC (Paraffin sections) (IHC-P). GTX109810
Anti-NPR-A antibody used in IHC (Paraffin sections) (IHC-P). GTX109810
Anti-NPR-A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109810

Cat. No. GTX109810

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Rabbit
Package
100 μl ($399),
25 μl ($169)
See all NPR-A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

119 kDa. ( Note )

Positive Control

HL-60 membrane extracts

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human NPR-A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951014

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

natriuretic peptide receptor 1 , ANPRA , ANPa , GUC2A , GUCY2A , NPRA

Cellular Localization

Membrane

Background

NPR1 is a membrane-bound guanylate cyclase that serves as the receptor for both atrial and brain natriuretic peptides (ANP (MIM 108780) and BNP (MIM 600295), respectively).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-NPR-A antibody used in Western Blot (WB). GTX109810

GTX109810 WB Image

Orthogonal

HL-60 whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NPR-A antibody (GTX109810) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 26098366 , PMID: 26269953

Anti-NPR-A antibody used in IHC (Paraffin sections) (IHC-P). GTX109810

GTX109810 IHC-P Image

NPR-A antibody detects NPR1 protein at on HBL435 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HBL435 xenograft.
NPR-A antibody (GTX109810) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NPR-A antibody used in IHC (Paraffin sections) (IHC-P). GTX109810

GTX109810 IHC-P Image

NPR-A antibody detects NPR-A protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
NPR-A antibody (GTX109810) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NPR-A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109810

GTX109810 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NPR-A (GTX109810) antibody at 1:200 dilution.

REFERENCE

Application Reference

Gu X et al. Biology (Basel) 2023; 12 (7) Corin Deficiency Diminishes Intestinal Sodium Excretion in Mice.
Gu, X et al. Biology 2023; 12 : 945 Corin Deficiency Diminishes Intestinal Sodium Excretion in Mice
Application : IHC-P
Reactivity : Mouse
Huang Y et al. Front Pharmacol 2023; 14 : 1290253 Investigating the role of NPR1 in dilated cardiomyopathy and its potential as a therapeutic target for glucocorticoid therapy.
Yang X et al. Med Sci Monit 2019; 25 : 9042-9047 Expression and Regulation of Brain Natriuretic Peptide and Natriuretic Peptide Receptor A (NPR-A) in L6-S1 Dorsal Root Ganglia in a Rat Model of Chronic Nonbacterial Prostatitis.
Application : WB
Reactivity : Rat
Lopez-Morales MA et al. Mol Cell Endocrinol 2018; 472 : 43109 Molecular mechanisms underlying the neuroprotective role of atrial natriuretic peptide in experimental acute ischemic stroke.
Application : WB
Reactivity : Rat
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169