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NRF2 antibody

Orthogonal
Anti-NRF2 antibody used in Western Blot (WB). GTX135165
Anti-NRF2 antibody used in Western Blot (WB). GTX135165
Anti-NRF2 antibody used in Western Blot (WB). GTX135165

Cat. No. GTX135165

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NRF2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.63 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of mouse NRF2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887459

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear factor, erythroid derived 2, like 2 , Nrf2

Cellular Localization

Cytoplasm, cytosol,Nucleus

Background

This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-NRF2 antibody used in Western Blot (WB). GTX135165

GTX135165 WB Image

Orthogonal

Untreated (–) and treated (+) Neuro2A whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody (GTX135165) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-NRF2 antibody used in Western Blot (WB). GTX135165

GTX135165 WB Image

Orthogonal

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody (GTX135165) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NRF2 antibody used in Western Blot (WB). GTX135165

GTX135165 WB Image

Orthogonal

Untreated (–) and treated (+) Rat2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody (GTX135165) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Gu X et al. Exp Ther Med 2023; 26 (3) : 413 1,25(OH)(2)D(3) ameliorates doxorubicin?induced cardiomyopathy by inhibiting the NLRP3 inflammasome and oxidative stress.
XIN GU et al. Exp Ther Med 2023; 26 : 413 1,25(OH)?D? ameliorates doxorubicin?induced cardiomyopathy by inhibiting the NLRP3 inflammasome and oxidative stress
Application : WB
Peng-Hsiang Fang et al. Mol Med 2022; 28 (1) : 71 Cobalt protoporphyrin promotes human keratinocyte migration under hyperglycemic conditions.
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169