APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:5000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
Based on internal testing result, this antibody does not cross react with human ENO1 and human ENO3.
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone AT17D10 is derived from hybridization of mouse F2 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human NSE protein.
Purification
Protein A Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
enolase 2 , ENO2 , HELS279 , NSE , Enolase 2 , Neuron Specific Enolase , gamma-Enolase
Cellular Localization
Cytoplasm,Cell membrane
Background
This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-NSE antibody [AT17D10] used in Western Blot (WB). GTX57594](/templates/default/js/lightbox/images/loading.gif "Anti-NSE antibody [AT17D10] used in Western Blot (WB). GTX57594")
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GTX57594 WB Image
WB analysis of various samples using GTX57594 NSE antibody.
Lane 1 : 293T cell lysate
Lane 2 : NSE transfected 293T cell lysate
Loading : 5 μg
Dilution : 1:1000
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GTX57594 WB Image
WB analysis of Mouse brain tissue lysate using GTX57594 NSE antibody.
Loading : 40 μg
Dilution : 1:500~1:5000
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GTX57594 ICC/IF Image
ICC/IF analysis of U87-MG cells using GTX57594 NSE antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100
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GTX57594 WB Image
WB analysis of various samples using GTX57594 NSE antibody.
Lane 1 : U87-MG whole cell lysate
Lane 2 : Jurkat whole cell lysate
Loading : 20 μg
Dilution : 1:1000
|
|
|
GTX57594 WB Image
WB analysis of various samples using GTX57594 NSE antibody.
Lane 1 : Recombinant Human ENO1 protein
Lane 2 : Recombinant Human NSE (ENO2) protein
Lane 3 : Recombinant Human ENO3 protein
Loading : 50 ng
Dilution : 1:1000
|
REFERENCE
There are currently no references for NSE antibody [AT17D10] (GTX57594). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for NSE antibody [AT17D10] (GTX57594). Be the first to share your experience with this product.
![Anti-NSE antibody [AT17D10] used in Western Blot (WB). GTX57594](/upload/website/prouct_img/small/GTX57594/GTX57594_20200204_WB2_w_23061123_236.webp "Anti-NSE antibody [AT17D10] used in Western Blot (WB). GTX57594")
![Anti-NSE antibody [AT17D10] used in Western Blot (WB). GTX57594](/upload/website/prouct_img/small/GTX57594/GTX57594_20200204_WB1_w_23061123_504.webp "Anti-NSE antibody [AT17D10] used in Western Blot (WB). GTX57594")
![Anti-NSE antibody [AT17D10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57594](/upload/website/prouct_img/small/GTX57594/GTX57594_20200204_IF_w_23061123_561.webp "Anti-NSE antibody [AT17D10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57594")
![Anti-NSE antibody [AT17D10] used in Western Blot (WB). GTX57594](/upload/website/prouct_img/small/GTX57594/GTX57594_20200204_WB3_w_23061123_824.webp "Anti-NSE antibody [AT17D10] used in Western Blot (WB). GTX57594")
![Anti-NSE antibody [AT17D10] used in Western Blot (WB). GTX57594](/upload/website/prouct_img/small/GTX57594/GTX57594_20200204_WB4_w_23061123_941.webp "Anti-NSE antibody [AT17D10] used in Western Blot (WB). GTX57594")
![Anti-NSE antibody [AT17D10] used in Western Blot (WB). GTX57594](/upload/website/prouct_img/normal/GTX57594/GTX57594_20200204_WB2_w_23061123_236.webp "Anti-NSE antibody [AT17D10] used in Western Blot (WB). GTX57594")
![Anti-NSE antibody [AT17D10] used in Western Blot (WB). GTX57594](/upload/website/prouct_img/normal/GTX57594/GTX57594_20200204_WB1_w_23061123_504.webp "Anti-NSE antibody [AT17D10] used in Western Blot (WB). GTX57594")
![Anti-NSE antibody [AT17D10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57594](/upload/website/prouct_img/normal/GTX57594/GTX57594_20200204_IF_w_23061123_561.webp "Anti-NSE antibody [AT17D10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57594")
![Anti-NSE antibody [AT17D10] used in Western Blot (WB). GTX57594](/upload/website/prouct_img/normal/GTX57594/GTX57594_20200204_WB3_w_23061123_824.webp "Anti-NSE antibody [AT17D10] used in Western Blot (WB). GTX57594")
![Anti-NSE antibody [AT17D10] used in Western Blot (WB). GTX57594](/upload/website/prouct_img/normal/GTX57594/GTX57594_20200204_WB4_w_23061123_941.webp "Anti-NSE antibody [AT17D10] used in Western Blot (WB). GTX57594")