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NSE2 antibody [6A6]

Anti-NSE2 antibody [6A6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83995
Anti-NSE2 antibody [6A6] used in Western Blot (WB). GTX83995
Anti-NSE2 antibody [6A6] used in Flow cytometry (FACS). GTX83995
Anti-NSE2 antibody [6A6] used in Flow cytometry (FACS). GTX83995

Cat. No. GTX83995

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6A6

Isotype

 IgG2a

Application

 WB, ICC/IF, FACS

Reactivity

 Human
Package
100 μl ($519)
See all NSE2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
FACS 1:100
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.52 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human NSMCE2 (NP_775956) produced in E.coli.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NSE2 (MMS21) homolog, SMC5-SMC6 complex SUMO ligase , C8orf36 , MMS21 , NSE2 , ZMIZ7

Cellular Localization

Nucleus

Background

E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. Acts as a E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, and maybe the cohesin components RAD21 and STAG2. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair.

Database

Research Area

DATA IMAGES

Anti-NSE2 antibody [6A6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83995

GTX83995 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with NSE2 plasmid using GTX83995 NSE2 antibody [6A6].

Anti-NSE2 antibody [6A6] used in Western Blot (WB). GTX83995

GTX83995 WB Image

WB analysis of HEK293T cells transfected with NSE2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83995 NSE2 antibody [6A6].
Loading : 5 ug per lane

Anti-NSE2 antibody [6A6] used in Flow cytometry (FACS). GTX83995

GTX83995 FACS Image

FACS analysis of Jurkat cells using GTX83995 NSE2 antibody [6A6].
Red : Primary antibody
Blue : Negative control antibody

Anti-NSE2 antibody [6A6] used in Flow cytometry (FACS). GTX83995

GTX83995 FACS Image

FACS analysis of HEK293T cells transfected with either NSE2 plasmid(Red) or empty vector control plasmid(Blue) using GTX83995 NSE2 antibody [6A6].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519