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NTCP antibody

Anti-NTCP antibody used in Western Blot (WB). GTX17693
Anti-NTCP antibody used in IHC (Paraffin sections) (IHC-P). GTX17693
Anti-NTCP antibody used in IHC (Paraffin sections) (IHC-P). GTX17693
Anti-NTCP antibody used in Flow cytometry (FACS). GTX17693
Anti-NTCP antibody used in IHC (Frozen sections) (IHC-Fr). GTX17693
Anti-NTCP antibody used in IHC (Paraffin sections) (IHC-P). GTX17693

Cat. No. GTX17693

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr, FACS

Reactivity

 Mouse, Rat
Package
100 μg ($429)
See all NTCP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.5μg/ml
IHC-P 0.5-1μg/ml
IHC-Fr 0.5-1μg/ml
FACS 1-3μg/1x10⁶cells
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.1% Na₂HPO₄, 0.45% NaCl, 2.5% BSA

Preservative

0.025% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

500 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse SLC10A1 (296-336aa EGLLFIIIFRCYLKIKPQKDQTKITYKAAATEDATPAALEK), different from the related human sequence by eighteen amino acids, and from the related rat sequence by three amino acids.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 10 (sodium/bile acid cotransporter family), member 1 , Ntcp

Cellular Localization

Membrane; Multi-pass membrane protein

Database

Research Area

DATA IMAGES

Anti-NTCP antibody used in Western Blot (WB). GTX17693

GTX17693 WB Image

WB analysis of various samples using GTX17693 NTCP antibody.
Lane 1: rat liver tissue lysates
Lane 2: mouse liver tissue lysates
Dilution: 0.5 μg/mL
Loading : 30μg of sample under reducing conditions

Anti-NTCP antibody used in IHC (Paraffin sections) (IHC-P). GTX17693

GTX17693 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX17693 NTCP antibody.
Antigen retrieval: Heat-mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution: 1μg/ml

Anti-NTCP antibody used in IHC (Paraffin sections) (IHC-P). GTX17693

GTX17693 IHC-P Image

IHC-P analysis of rat liver tissue using GTX17693 NTCP antibody.
Antigen retrieval: Heat-mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) for 20 mins
Dilution: 1μg/ml

Anti-NTCP antibody used in Flow cytometry (FACS). GTX17693

GTX17693 FACS Image

FACS analysis of HEPA1-6 cell using GTX17693 NTCP antibody.
Blue: Primary antibody
Green: Isotype control
Red: Cell-only control
Antibody amount : 1μg/1x10⁶ cells for 30 min at 20ºC

Anti-NTCP antibody used in IHC (Frozen sections) (IHC-Fr). GTX17693

GTX17693 IHC-Fr Image

IHC-Fr analysis of mouse liver tissue using GTX17693 NTCP antibody.
Antigen retrieval: Heat-mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) for 20 mins
Dilution: 1μg/ml

Anti-NTCP antibody used in IHC (Paraffin sections) (IHC-P). GTX17693

GTX17693 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX17693 NTCP antibody.
Antigen retrieval: Heat-mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) for 20 mins
Dilution: 1μg/ml

REFERENCE

Application Reference

Murphy WA et al. Hepatol Commun 2024; 8 (3) Alterations in zonal distribution and plasma membrane localization of hepatocyte bile acid transporters in patients with NAFLD.
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 429