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NTR1 antibody

Anti-NTR1 antibody used in Western Blot (WB). GTX02541
Anti-NTR1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX02541
Anti-NTR1 antibody used in Flow cytometry (FACS). GTX02541
Anti-NTR1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02541
Anti-NTR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02541

Cat. No. GTX02541

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS

Reactivity

Human, Rat
Package
50 μl ($579)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide EQNRSADGQHAGGLVC corresponding to amino acid residues 209-224 of human NTSR1 (2nd extracellular loop).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neurotensin receptor 1 , NTR

Cellular Localization

Cell membrane

Background

Neurotensin receptor 1 belongs to the large superfamily of G-protein coupled receptors. NTSR1 mediates the multiple functions of neurotensin, such as hypotension, hyperglycemia, hypothermia, antinociception, and regulation of intestinal motility and secretion. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-NTR1 antibody used in Western Blot (WB). GTX02541

GTX02541 WB Image

WB analysis of HT-29 (lanes 1 and 4), NCI-H526 (lanes 2 and 5), and MDA-MB-468 (lanes 3 and 6) cell lysates using GTX02541 NTR1 antibody.
Lane 1-3 : Primary antibody
Lane 4-6 : Primary antibody prior incubated with immunogen peptide
Dilution : 1:200

Anti-NTR1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX02541

GTX02541 IHC-Fr Image

IHC-Fr analysis of rat brain tissue sections using GTX02541 NTR1 antibody. Arrows point at examples of colocalization.
Green : NTR1 antibody
Red : Choline acetyltransferase (ChAT) antibody
Blue : DAPI
Dilution : 1:100

Anti-NTR1 antibody used in Flow cytometry (FACS). GTX02541

GTX02541 FACS Image

FACS analysis of live intact HL-60 cells using GTX02541 NTR1 antibody.
Green : Primary antibody
Black : Secondary antibody only control
Dilution : 1:20

Anti-NTR1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02541

GTX02541 ICC/IF Image

Live cell imaging analysis of MCF-7 cells using GTX02541 NTR1 antibody.
Panel A : Primary antibody
Panel B : Live view of the cell
Panel C : Merged images of Panel A and B
Dilution : 1:20

Anti-NTR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02541

GTX02541 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX02541 NTR1 antibody. Staining (brown color) is specific for epithelium-derivated malignant cells.
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 579