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NUF2 antibody

Orthogonal
Anti-NUF2 antibody used in Western Blot (WB). GTX110734
Anti-NUF2 antibody used in Western Blot (WB). GTX110734
Anti-NUF2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110734
Anti-NUF2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110734
Anti-NUF2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110734

Cat. No. GTX110734

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all NUF2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.52 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein corresponding to human NUF2.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951038

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NUF2 component of NDC80 kinetochore complex , CDCA1 , CT106 , NUF2R

Cellular Localization

Nucleus , Kinetochore

Background

This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NUF2 antibody used in Western Blot (WB). GTX110734

GTX110734 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NUF2 antibody (GTX110734) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-NUF2 antibody used in Western Blot (WB). GTX110734

GTX110734 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NUF2 antibody (GTX110734) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NUF2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110734

GTX110734 ICC/IF Image

NUF2 antibody detects NUF2 protein at kinetochore by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NUF2 stained by NUF2 antibody (GTX110734) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NUF2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110734

GTX110734 ICC/IF Image

CDCA1 antibody [GT312] detects CDCA1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CDCA1 protein stained by CDCA1 antibody [GT312] (GTX110734) diluted at 1:200.
Blue: Hoechst 33342 staining.

Anti-NUF2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110734

GTX110734 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using CDCA1(GTX110734) antibody (Geen) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169