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NUP155 antibody [C1C3]

Anti-NUP155 antibody [C1C3] used in Western Blot (WB). GTX120945
Anti-NUP155 antibody [C1C3] used in Western Blot (WB). GTX120945

Cat. No. GTX120945

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all NUP155 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

155 kDa. ( Note )

Positive Control

NT2D1 , 293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human NUP155. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10721585

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nucleoporin 155 , ATFB15 , N155

Background

Nucleoporins are the main components of the nuclear pore complex (NPC) of eukaryotic cells. They are involved in the bidirectional trafficking of molecules, especially mRNAs and proteins, between the nucleus and the cytoplasm. The protein encoded by this gene does not contain the typical FG repeat sequences found in most vertebrate nucleoporins. Two protein isoforms are encoded by transcript variants of this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NUP155 antibody [C1C3] used in Western Blot (WB). GTX120945

GTX120945 WB Image

NUP155 antibody detects NUP155 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NUP155 antibody (GTX120945) diluted by 1:1000.

Anti-NUP155 antibody [C1C3] used in Western Blot (WB). GTX120945

GTX120945 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
5% SDS PAGE
GTX120945 diluted at 1:1000

REFERENCE

Application Reference

LaJoie D et al. Mol Biol Cell 2022; 33 (13) : ar117 A role for Nup153 in nuclear assembly reveals differential requirements for targeting of nuclear envelope constituents.
Application : WB
Reactivity : Human
Aslanian A et al. DNA Repair (Amst) 2014; 15 (Epub) : 29-38 Mass spectrometry-based quantification of the cellular response to methyl methanesulfonate treatment in human cells.
Application : WB
Reactivity : Human
Chow KH et al. Mol Biol Cell 2014; 25 (1) : 160-8 The SUMO proteases SENP1 and SENP2 play a critical role in nucleoporin homeostasis and nuclear pore complex function.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169