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Nanos3 antibody

Anti-Nanos3 antibody used in IHC (Paraffin sections) (IHC-P). GTX85389
Anti-Nanos3 antibody used in IHC (Paraffin sections) (IHC-P). GTX85389
Anti-Nanos3 antibody used in Western Blot (WB). GTX85389

Cat. No. GTX85389

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all NANOS3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

Human Brain Tissue Slide(GTX22203) , Human Brain Tissue Lysate(GTX27918)

Product Note

This Nanos3 antibody will not cross-react with either Nanos 1 or Nanos2.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Nanos3 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of the human Nanos3.The immunogen is located within the last 50 amino acids of Nanos3.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nanos C2HC-type zinc finger 3 , NANOS1L , NOS3 , ZC2HC12C

Cellular Localization

Nucleus,Cytoplasm

Background

Nanos is a zinc-finger containing, RNA-binding protein that has been implicated in germ cell development in both invertebrates and vertebrates. In drosophila, Nanos represses apoptosis during development to ensure proper germ-line development. Unlike Nanos1 whose expression in mice is dispensable, the Nanos2 and Nanos3 proteins are required for germ cell development. Nanos2- primordial germ cells (PGCs) die only in the male gonads and show no defects in females, while Nanos3- PGCs are lost during the migration stage regardless of sex. Nanos2 and Nanos3 have distinct expression patterns during embryo development, suggesting that these two proteins do not have redundant functions. However, expression of Nanos2 can at least partially replace Nanos3 function in a Nanos3- background. Nanos3 expression can not rescue Nanos2- defects. This Nanos3 antibody will not cross-react with either Nanos 1 or Nanos2.

Database

Research Area

DATA IMAGES

Anti-Nanos3 antibody used in IHC (Paraffin sections) (IHC-P). GTX85389

GTX85389 IHC-P Image

IHC-P analysis of human brain tissue using GTX85389 Nanos3 antibody.
Working concentration : 20 μg/ml

Anti-Nanos3 antibody used in IHC (Paraffin sections) (IHC-P). GTX85389

GTX85389 IHC-P Image

IHC-P analysis of human brain tissue using GTX85389 Nanos3 antibody.
Working concentration : 2.5 μg/ml

Anti-Nanos3 antibody used in Western Blot (WB). GTX85389

GTX85389 WB Image

WB analysis of human brain tissue lysate using GTX85389 Nanos3 antibody.
Working concentration : 2 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399