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Necdin antibody

KO/KD
Anti-Necdin antibody used in IHC (Frozen sections) (IHC-Fr). GTX00751
Anti-Necdin antibody used in IHC (Frozen sections) (IHC-Fr). GTX00751
Anti-Necdin antibody used in Western Blot (WB). GTX00751

Cat. No. GTX00751

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr, IP, ChIP assay, Purification

Reactivity

 Human, Mouse, Rat, Chicken
Package
100 μg ($399)
See all Necdin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:3000
ICC/IF 1:500
IHC-Fr 1:500
IP 1:100
ChIP assay 1:100
Purification Assay dependent

Note :

Purification
Identification of Necdin interacting proteins by column conjugated with anti-Necdin antibody.
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant GST-fused mouse necdin (aa 83-325)

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

necdin , AI528698 , Peg6

Cellular Localization

Cytoplasm,nucleoplasm

Database

Research Area

DATA IMAGES

Anti-Necdin antibody used in IHC (Frozen sections) (IHC-Fr). GTX00751

GTX00751 IHC-Fr Image

KO/KD

IHC-Fr analysis of mouse cervical dorsal root ganglion tissue section (E14.5) using GTX00751 Necdin antibody.
Dilution : 1:500

Anti-Necdin antibody used in IHC (Frozen sections) (IHC-Fr). GTX00751

GTX00751 IHC-Fr Image

IHC-Fr analysis of mouse forebrain tissue section (E13.5) using GTX00751 Necdin antibody.
CX : Cortex
LV : Lateral Ventricle
LGE : Lateral Ganglionic Eminence
SP : Septum
Dilution : 1:500

Anti-Necdin antibody used in Western Blot (WB). GTX00751

GTX00751 WB Image

WB analysis of mouse embryo (E16.5) using GTX00751 Necdin antibody. The larger size reported here and elsewhere may reflect post-translational modifications such as ubiquitination and phosphorylation at several sites.
Dilution : 1:3000
Loading : 20 μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399