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Nek1 antibody

KO/KD Orthogonal
Anti-Nek1 antibody used in Western Blot (WB). GTX130828
Anti-Nek1 antibody used in Western Blot (WB). GTX130828
Anti-Nek1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130828
Anti-Nek1 antibody used in Western Blot (WB). GTX130828
Anti-Nek1 antibody used in Western Blot (WB). GTX130828
Anti-Nek1 antibody used in Western Blot (WB). GTX130828

Cat. No. GTX130828

Reference 1
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Nek1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

143 kDa. ( Note )

Observed MW

180 kDa. The observed M.W. is based on the following publication. PMID: 16603261

Positive Control

293T , mouse brain , rat brain

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Nek1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886357

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NIMA related kinase 1 , ALS24 , NY-REN-55 , SRPS2 , SRPS2A , SRTD6

Cellular Localization

Nucleus , Cytoplasm

Background

The protein encoded by this gene is a serine/threonine kinase involved in cell cycle regulation. The encoded protein is found in a centrosomal complex with FEZ1, a neuronal protein that plays a role in axonal development. Defects in this gene are a cause of polycystic kidney disease (PKD). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2010]

Database

Research Area

DATA IMAGES

Anti-Nek1 antibody used in Western Blot (WB). GTX130828

GTX130828 WB Image

KO/KD

Wild-type (WT) and NEK1 knockout (KO) HeLa cell extracts (100 μg) were separated by gradient gel% SDS-PAGE, and the membrane was blotted with Nek1 antibody (GTX130828) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.

Anti-Nek1 antibody used in Western Blot (WB). GTX130828

GTX130828 WB Image

Orthogonal

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Nek1 antibody (GTX130828) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Nek1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130828

GTX130828 ICC/IF Image

Nek1 antibody detects Nek1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nek1 protein stained by Nek1 antibody (GTX130828) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Nek1 antibody used in Western Blot (WB). GTX130828

GTX130828 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Nek1 antibody (GTX130828) diluted at 1:1000.
The observed M.W. is based on the publication: PMID: 16603261

Anti-Nek1 antibody used in Western Blot (WB). GTX130828

GTX130828 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Nek1 antibody (GTX130828) diluted at 1:1000.

Anti-Nek1 antibody used in Western Blot (WB). GTX130828

GTX130828 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Nek1 antibody (GTX130828) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

Application Reference

Freund I et al. Cells 2020; 9 (5) Fractionation-Dependent Radiosensitization by Molecular Targeting of Nek1.
Submit a Reference

REVIEW

Nek1 antibody Cat. No. GTX130828

Rating
( Average 5 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Immunoprecipitation(IP)
( Average 5 based on 1 users reviews)
Anti-Nek1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130828
Date : Anonymous submitted on 09-Jun-2022
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : HeLa WT and NEK1 KO cells
Blocking : 5% BSA, 5% goat serum and 0.01% Triton X-100 in PBS, 30min, RT
Fixation : 4% PFA in PBS, 15 min, RT
Permeabilization /Antigen retrieval : 0.1% Triton X-100 in PBS, 10 min, RT
Primary Antibody : 1:300, 4°C, > 12Hr
Lane Description :
Yellow dashed line: HeLa WT cells
Magenta dashed line: HeLa KO cells
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-Nek1 antibody used in Immunoprecipitation (IP). GTX130828
Date : Anonymous submitted on 09-Jun-2022
Score :
Application Tested : IP
Sample Species : Hu
Sample : HeLa cells
Primary Antibody : Undiluted, 4°C, > 12Hr
Lane Description :
SM: 10% starting material
UB: 10% unbound fraction
IP: IP antibody
Figure Note : Antibody-bead conjugates were prepared by adding 1.0 μg of antibody to 500 μl of PBS with 0.01% triton X-100, together with 30μl of Dynabeads Protein A-Sepharose beads. One ml aliquots at 1.0 mg/ml of lysate were incubated with an antibody-bead conjugate for ~2 hrs at 4ºC.
Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-Nek1 antibody used in Western Blot (WB). GTX130828
Date : Anonymous submitted on 09-Jun-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa WT and NEK1 KO cells
Amount used : 100μg
Primary Antibody : 1:1000, 4°C, > 12Hr
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169