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Nestin antibody

Anti-Nestin antibody used in Immunohistochemistry (IHC). GTX85457

Cat. No. GTX85457

Reference 1

Host

 Chicken

Clonality

 Polyclonal

Isotype

 IgY

Application

 WB, ICC/IF, IHC

Reactivity

 Mouse
Package
50 μg ($399)
See all Nestin products

APPLICATION

Application Note

Recommended starting dilutions:
For IHC: Use at 1:3000 - 1:6000.
For ICC/IF:Use at 1:3000 - 1:6000.
For WB: Use at 1:5000 - 1:10000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Calculated MW

177 kDa. ( Note )

Product Note

Nestin

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Peptides

Purification

Affinity purified antibody

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nestin , Nbla00170

Background

This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-Nestin antibody used in Immunohistochemistry (IHC). GTX85457

GTX85457 IHC Image

IHC analysis of E13.5 mouse brain using Nestin antibody (green) and DAPI (blue).

REFERENCE

Application Reference

Mizukoshi T et al. eNeuro 2023; 10 (10) Spatiotemporal Regulation of De Novo and Salvage Purine Synthesis during Brain Development.
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399