Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.25-1μg/ml |
4μg antibody/ 500μg lysate |
Not tested in other applications.
Calculated MW
Product Note
Recognizes rat spinophilin at ~120kD. Also recognizes a band at ~95kD which is suspected to be a cleavage product of spinophilin.
Form
Liquid
Buffer
0.1M Tris-Glycine pH7.4, 0.15M Sodium Chloride, 30% Glycerol
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Rat
Immunogen
GST fusion protein corresponding to residues 286-390 of rat brain Spinophilin.
Purification
Protein G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Cellular Localization
Cytoplasm,Cytoskeleton,Nucleus,Cell junction,Synapse,Adherens junction,Cell projection,Dendritic spine,Cell membrane,Lamellipodium,Filopodium,Ruffle membrane
Background
interacts with protein phosphatase 1 and actin filaments; involved in regulation of synaptic transmission [RGD, Feb 2006]
Database
Research Area