APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Mouse brain , human brain , U87MG conditioned medium , rat brain
Predict Reactivity
Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.96 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the Extracellular domain of human Neuregulin-1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10619413
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
neuregulin 1 , ARIA , GGF , GGF2 , HGL , HRG , HRG1 , HRGA , MST131 , MSTP131 , NDF , NRG1-IT2 , SMDF
Cellular Localization
Pro-neuregulin-1 , membrane-bound isoform: Cell membrane; Single-pass type I membrane protein , Neuregulin-1: Secreted , Isoform 8: Nucleus , Isoform 9: Secreted , Isoform 10: Membrane; Single-pass type I membrane protein
Background
Neuregulin 1 (NRG1) was originally identified as a 44-kD glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. It is known that an extraordinary variety of different isoforms are produced from the NRG1 gene by alternative splicing. These isoforms include heregulins (HRGs), glial growth factors (GGFs) and sensory and motor neuron-derived factor (SMDF). They are tissue-specifically expressed and differ significantly in their structure. The HRG isoforms all contain immunoglobulin (Ig) and epidermal growth factor-like (EGF-like) domains. GGF and GGF2 isoforms contain a kringle-like sequence plus Ig and EGF-like domains; and the SMDF isoform shares only the EGF-like domain with other isoforms. The receptors for all NRG1 isoforms are the ERBB family of tyrosine kinase transmembrane receptors. Through interaction with ERBB receptors, NRG1 isoforms induce the growth and differentialtion of epithelial, neuronal, glial, and other types of cells. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX101117 WB Image
Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Neuregulin-1 antibody (GTX101117) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101117 ICC/IF Image
Neuregulin-1 antibody detects Neuregulin-1 protein at nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Neuregulin-1 protein stained by Neuregulin-1 antibody (GTX101117) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Blue: Hoechst 33342 staining.
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GTX101117 WB Image
Sample (50 μg of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX101117 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101117 WB Image
Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Neuregulin-1 antibody (GTX101117) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101117 WB Image
U87-MG whole cell extract and conditioned medium (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Neuregulin-1 antibody (GTX101117) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101117 WB Image
The data was published in the journal Sci Rep in 2017.PMID: 28751722
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GTX101117 WB Image
The data was published in the journal Sci Rep in 2017.PMID: 28751722
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GTX101117 WB Image
The data was published in the journal Sci Rep in 2017.PMID: 28751722
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REFERENCE
REVIEW
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