APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:300-1:5000 |
| 1:200-1:400 |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
1% BSA, 50% glycerol
Preservative
0.09% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
KLH conjugated synthetic peptide derived from human Neutrophil Elastase (101-200).
Purification
Protein A purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
elastase, neutrophil expressed , ELA2 , GE , HLE , HNE , NE , PMN-E , SCN1
Cellular Localization
Phagosome
Background
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19. [provided by RefSeq, Jan 2016]
Database
Research Area
DATA IMAGES
|
GTX03641 WB Image
WB analysis of various sample lysates using GTX03641 Neutrophil elastase antibody.
Lane 1 : Mouse Bone tissue lysates
Lane 2 : Rat Bone tissue lysates
Dilution : 1:1000
|
|
|
GTX03641 WB Image
WB analysis of mouse spinal cord lysates using GTX03641 Neutrophil elastase antibody.
Dilution : 1:300
|
|
|
GTX03641 FACS Image
FACS analysis of A549 cells using GTX03641 Neutrophil elastase antibody.
Green : Primary antibody
Orange : Isotype control
Blue : Control cells
Light blue : Secondary Ab only
Dilution : 1:100
|
|
|
GTX03641 WB Image
WB analysis of HL-60 cell lysates using GTX03641 Neutrophil elastase antibody.
Dilution : 1:300
|
|
|
GTX03641 FACS Image
FACS analysis of 4% PFA-fixed THP-1 cells using GTX03641 Neutrophil elastase antibody. Acquisitions of 20,000 events were performed.
Green : Primary antibody
Orange : Isotype control
Dilution : 1:100
Permeabilization : 0.1%PBST for 20 min at room temperature
|
REFERENCE
There are currently no references for Neutrophil elastase antibody (GTX03641). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Neutrophil elastase antibody (GTX03641). Be the first to share your experience with this product.
