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Nitrotyrosine antibody [HM.11]

Cat. No. GTX54508

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 HM.11

Isotype

 IgG2b

Application

 WB, IHC-P, IHC-Fr, ELISA

Reactivity

 Species independent
Package
50 μg ($399)
See all Nitrotyrosine products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1:200-1:400
IHC-Fr Assay dependent
ELISA Assay dependent

Note :

IHC-P
10% formalin fixation; 3% H2O2 to block endogenous peroxidases; Citrate buffer pH 6.0 for 1 min at 100ºC as antigen retrieval treatment

IHC-Fr
also cytospins: acetone fixation 10 min -20 °C; block endogenous peroxidase by 0.3 % H2O2 in PBS (or methanol for intracellular staining); blocking with 10% NGS or 5 % BSA for 30 min. use at assay dependent concentration
Not tested in other applications.

Product Note

The monoclonal antibody HM.11 recognizes nitrotyrosine, both with the free amino acid as well as with proteins containing nitrotyrosine

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 0.1% BSA

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

100 μg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Nitrated KLH

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

REFERENCE

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REVIEW

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Package List Price ($)
$ 399