APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml |
| 1-2μg/ml |
| 1-2μg/ml for 30 minutes at RT |
| 1-2μg/10⁶ cells |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
Product Note
We do not recommend use of this product for Mouse,Rat,Bovine samples.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant full-length human NCL protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
C23 , NCL , nucleolin , Nucleolin
Cellular Localization
Nucleus,Nucleolus,Cytoplasm
Background
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-Nucleolin antibody [NCL/902] used in IHC (Paraffin sections) (IHC-P). GTX34914](/templates/default/js/lightbox/images/loading.gif "Anti-Nucleolin antibody [NCL/902] used in IHC (Paraffin sections) (IHC-P). GTX34914")
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GTX34914 IHC-P Image
IHC-P analysis of human skin tissue using GTX34914 Nucleolin antibody [NCL/902].
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GTX34914 WB Image
WB analysis of A431 cell lysate using GTX34914 Nucleolin antibody [NCL/902].
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GTX34914 FACS Image
FACS analysis of Jurkat cells using GTX34914 Nucleolin antibody [NCL/902].
Grey : Isotype Control
Other : Primary antibody
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GTX34914 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX34914 Nucleolin antibody [NCL/902].
Green : Primary antibody
Blue : nucleus
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GTX34914 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX34914 Nucleolin antibody [NCL/902].
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GTX34914 IHC-P Image
IHC-P analysis of human bladder carcinoma tissue using GTX34914 Nucleolin antibody [NCL/902].
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GTX34914 ICC/IF Image
ICC/IF analysis of PFA-fixed MCF-7 cells using GTX34914 Nucleolin antibody [NCL/902].
Green : Primary antibody
Red : Phalloidin
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GTX34914 ICC/IF Image
ICC/IF analysis of PFA-fixed K562 cells using GTX34914 Nucleolin antibody [NCL/902].
Green : Primary antibody
Red : Phalloidin
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GTX34914 IHC-P Image
IHC-P analysis of human uterus tissue using GTX34914 Nucleolin antibody [NCL/902].
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GTX34914 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX34914 Nucleolin antibody [NCL/902].
Green : Primary antibody
Blue : nucleus
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GTX34914 FACS Image
FACS analysis of 293T cells using GTX34914 Nucleolin antibody [NCL/902].
Black : Blank
Grey : Isotype Control
Green : Primary antibody
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GTX34914 IHC-P Image
IHC-P analysis of human ovarian carcinoma tissue using GTX34914 Nucleolin antibody [NCL/902].
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REFERENCE
There are currently no references for Nucleolin antibody [NCL/902] (GTX34914). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Nucleolin antibody [NCL/902] (GTX34914). Be the first to share your experience with this product.
![Anti-Nucleolin antibody [NCL/902] used in IHC (Paraffin sections) (IHC-P). GTX34914](/upload/website/prouct_img/small/GTX34914/GTX34914_20200115_IHC-P_1135_w_23060801_542.webp "Anti-Nucleolin antibody [NCL/902] used in IHC (Paraffin sections) (IHC-P). GTX34914")
![Anti-Nucleolin antibody [NCL/902] used in Western Blot (WB). GTX34914](/upload/website/prouct_img/small/GTX34914/GTX34914_20200115_WB_40_w_23060801_417.webp "Anti-Nucleolin antibody [NCL/902] used in Western Blot (WB). GTX34914")
![Anti-Nucleolin antibody [NCL/902] used in Flow cytometry (FACS). GTX34914](/upload/website/prouct_img/small/GTX34914/GTX34914_20200115_FACS_1483_w_23060801_523.webp "Anti-Nucleolin antibody [NCL/902] used in Flow cytometry (FACS). GTX34914")
![Anti-Nucleolin antibody [NCL/902] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34914](/upload/website/prouct_img/small/GTX34914/GTX34914_20200115_ICC-IF_197_w_23060801_803.webp "Anti-Nucleolin antibody [NCL/902] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34914")
![Anti-Nucleolin antibody [NCL/902] used in IHC (Paraffin sections) (IHC-P). GTX34914](/upload/website/prouct_img/small/GTX34914/GTX34914_20200115_IHC-P_1391_w_23060801_113.webp "Anti-Nucleolin antibody [NCL/902] used in IHC (Paraffin sections) (IHC-P). GTX34914")
![Anti-Nucleolin antibody [NCL/902] used in IHC (Paraffin sections) (IHC-P). GTX34914](/upload/website/prouct_img/small/GTX34914/GTX34914_20200115_IHC-P_307_w_23060801_837.webp "Anti-Nucleolin antibody [NCL/902] used in IHC (Paraffin sections) (IHC-P). GTX34914")
![Anti-Nucleolin antibody [NCL/902] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34914](/upload/website/prouct_img/small/GTX34914/GTX34914_20200115_ICC-IF_1737_w_23060801_603.webp "Anti-Nucleolin antibody [NCL/902] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34914")
![Anti-Nucleolin antibody [NCL/902] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34914](/upload/website/prouct_img/small/GTX34914/GTX34914_20200115_ICC-IF_1715_w_23060801_163.webp "Anti-Nucleolin antibody [NCL/902] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34914")
![Anti-Nucleolin antibody [NCL/902] used in IHC (Paraffin sections) (IHC-P). GTX34914](/upload/website/prouct_img/small/GTX34914/GTX34914_20200115_IHC-P_1440_w_23060801_989.webp "Anti-Nucleolin antibody [NCL/902] used in IHC (Paraffin sections) (IHC-P). GTX34914")
![Anti-Nucleolin antibody [NCL/902] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34914](/upload/website/prouct_img/small/GTX34914/GTX34914_20200115_ICC-IF_227_w_23060801_959.webp "Anti-Nucleolin antibody [NCL/902] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34914")
![Anti-Nucleolin antibody [NCL/902] used in Flow cytometry (FACS). GTX34914](/upload/website/prouct_img/small/GTX34914/GTX34914_20200115_FACS_29_w_23060801_643.webp "Anti-Nucleolin antibody [NCL/902] used in Flow cytometry (FACS). GTX34914")
![Anti-Nucleolin antibody [NCL/902] used in IHC (Paraffin sections) (IHC-P). GTX34914](/upload/website/prouct_img/small/GTX34914/GTX34914_20200115_IHC-P_830_w_23060801_897.webp "Anti-Nucleolin antibody [NCL/902] used in IHC (Paraffin sections) (IHC-P). GTX34914")
![Anti-Nucleolin antibody [NCL/902] used in IHC (Paraffin sections) (IHC-P). GTX34914](/upload/website/prouct_img/normal/GTX34914/GTX34914_20200115_IHC-P_1135_w_23060801_542.webp "Anti-Nucleolin antibody [NCL/902] used in IHC (Paraffin sections) (IHC-P). GTX34914")
![Anti-Nucleolin antibody [NCL/902] used in Western Blot (WB). GTX34914](/upload/website/prouct_img/normal/GTX34914/GTX34914_20200115_WB_40_w_23060801_417.webp "Anti-Nucleolin antibody [NCL/902] used in Western Blot (WB). GTX34914")
![Anti-Nucleolin antibody [NCL/902] used in Flow cytometry (FACS). GTX34914](/upload/website/prouct_img/normal/GTX34914/GTX34914_20200115_FACS_1483_w_23060801_523.webp "Anti-Nucleolin antibody [NCL/902] used in Flow cytometry (FACS). GTX34914")
![Anti-Nucleolin antibody [NCL/902] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34914](/upload/website/prouct_img/normal/GTX34914/GTX34914_20200115_ICC-IF_197_w_23060801_803.webp "Anti-Nucleolin antibody [NCL/902] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34914")
![Anti-Nucleolin antibody [NCL/902] used in IHC (Paraffin sections) (IHC-P). GTX34914](/upload/website/prouct_img/normal/GTX34914/GTX34914_20200115_IHC-P_1391_w_23060801_113.webp "Anti-Nucleolin antibody [NCL/902] used in IHC (Paraffin sections) (IHC-P). GTX34914")
![Anti-Nucleolin antibody [NCL/902] used in IHC (Paraffin sections) (IHC-P). GTX34914](/upload/website/prouct_img/normal/GTX34914/GTX34914_20200115_IHC-P_307_w_23060801_837.webp "Anti-Nucleolin antibody [NCL/902] used in IHC (Paraffin sections) (IHC-P). GTX34914")
![Anti-Nucleolin antibody [NCL/902] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34914](/upload/website/prouct_img/normal/GTX34914/GTX34914_20200115_ICC-IF_1737_w_23060801_603.webp "Anti-Nucleolin antibody [NCL/902] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34914")
![Anti-Nucleolin antibody [NCL/902] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34914](/upload/website/prouct_img/normal/GTX34914/GTX34914_20200115_ICC-IF_1715_w_23060801_163.webp "Anti-Nucleolin antibody [NCL/902] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34914")
![Anti-Nucleolin antibody [NCL/902] used in IHC (Paraffin sections) (IHC-P). GTX34914](/upload/website/prouct_img/normal/GTX34914/GTX34914_20200115_IHC-P_1440_w_23060801_989.webp "Anti-Nucleolin antibody [NCL/902] used in IHC (Paraffin sections) (IHC-P). GTX34914")
![Anti-Nucleolin antibody [NCL/902] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34914](/upload/website/prouct_img/normal/GTX34914/GTX34914_20200115_ICC-IF_227_w_23060801_959.webp "Anti-Nucleolin antibody [NCL/902] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34914")
![Anti-Nucleolin antibody [NCL/902] used in Flow cytometry (FACS). GTX34914](/upload/website/prouct_img/normal/GTX34914/GTX34914_20200115_FACS_29_w_23060801_643.webp "Anti-Nucleolin antibody [NCL/902] used in Flow cytometry (FACS). GTX34914")
![Anti-Nucleolin antibody [NCL/902] used in IHC (Paraffin sections) (IHC-P). GTX34914](/upload/website/prouct_img/normal/GTX34914/GTX34914_20200115_IHC-P_830_w_23060801_897.webp "Anti-Nucleolin antibody [NCL/902] used in IHC (Paraffin sections) (IHC-P). GTX34914")