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Nucleophosmin antibody [7H10B9]

Anti-Nucleophosmin antibody [7H10B9] used in Western Blot (WB). GTX83217
Anti-Nucleophosmin antibody [7H10B9] used in IHC (Paraffin sections) (IHC-P). GTX83217
Anti-Nucleophosmin antibody [7H10B9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83217

Cat. No. GTX83217

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 7H10B9

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Monkey
Package
100 μl ($399)
See all Nucleophosmin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human Nucleophosmin (2-265) expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nucleophosmin 1 , B23 , NPM

Cellular Localization

nucleoplasm,Nucleolus

Background

This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Nov 2009]

Database

Research Area

DATA IMAGES

Anti-Nucleophosmin antibody [7H10B9] used in Western Blot (WB). GTX83217

GTX83217 WB Image

WB analysis of SMMC-7721 (1), HepG2 (2), HeLa (3) and HEK293 (4) cell lysate using GTX83217 Nucleophosmin antibody [7H10B9].

Anti-Nucleophosmin antibody [7H10B9] used in IHC (Paraffin sections) (IHC-P). GTX83217

GTX83217 IHC-P Image

IHC-P analysis of human liver carcinoma tissue using GTX83217 Nucleophosmin antibody [7H10B9].

Anti-Nucleophosmin antibody [7H10B9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83217

GTX83217 ICC/IF Image

ICC/IF analysis of HeLa (left) and NTERA-2 (right) cells using GTX83217 Nucleophosmin antibody [7H10B9].
Green : Nucleophosmin
Red: Actin filaments

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399