APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.03-0.1μg/ml |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89581-PEP).
Product Note
This antibody is expected to recognise both report isoforms (NP_002511.1; NP_954654.1).
Predict Reactivity
Rat, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-QEAIQDLWQWRKSL, from the C Terminus of the protein sequence according to NP_002511.1; NP_954654.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
nucleophosmin 1 , B23 , NPM
Cellular Localization
nucleoplasm , Nucleolus
Background
The protein encoded by this gene is involved in several cellular processes, including centrosome duplication, protein chaperoning, and cell proliferation. The encoded phosphoprotein shuttles between the nucleolus, nucleus, and cytoplasm, chaperoning ribosomal proteins and core histones from the nucleus to the cytoplasm. This protein is also known to sequester the tumor suppressor ARF in the nucleolus, protecting it from degradation until it is needed. Mutations in this gene are associated with acute myeloid leukemia. Dozens of pseudogenes of this gene have been identified. [provided by RefSeq, Aug 2017]
Database
Research Area
DATA IMAGES
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GTX89581 ICC/IF Image
ICC/IF analysis of PFA fixed HeLa cells using GTX89581 Nucleophosmin antibody, C-term.
Green : Primary antibody
Blue : DAPI
Permeabilization : 0.15% Triton
Dilution : 10μg/ml
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GTX89581 WB Image
WB analysis of NIH3T3 (A), Daudi (B), Jurkat (C) and K562 (D) cell lysate (35μg protein in RIPA buffer) using GTX89581 Nucleophosmin antibody, C-term.
Dilution : 1μg/ml (NIH3T3), 0.1μg/ml (Daudi and Jurkat), and 0.01μg/ml (K562)
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GTX89581 WB Image
WB analysis of mouse spleen lysate (35μg protein in RIPA buffer) using GTX89581 Nucleophosmin antibody, C-term.
Dilution : 1μg/ml
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GTX89581 IHC-P Image
IHC-P analysis of paraffin embedded human testis using GTX89581 Nucleophosmin antibody, C-term.
Antigen retrieval : Steamed antigen retrieval with citrate buffer pH 6
Dilution : 5μg/ml
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REFERENCE
There are currently no references for Nucleophosmin antibody, C-term (GTX89581). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Nucleophosmin antibody, C-term (GTX89581). Be the first to share your experience with this product.
