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Nucleoporin p62 antibody [2A11]

Anti-Nucleoporin p62 antibody [2A11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00696
Anti-Nucleoporin p62 antibody [2A11] used in Western Blot (WB). GTX00696

Cat. No. GTX00696

Reference 2

Host

 Rat

Clonality

 Monoclonal

Clone Name

 2A11

Isotype

 IgG1

Application

 WB, ICC/IF, IP, ELISA

Reactivity

 Human, Monkey
Package
200 μg ($399)
See all Nucleoporin p62 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:400
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Nup62 (aa 1-300) (GST-Nup62-His).
Epitope: 1-179 amino acids(FG-repeat region)

Purification

Purified IgG
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nucleoporin 62 , IBSN , SNDI , p62

Background

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Nucleoporin p62 antibody [2A11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00696

GTX00696 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX00696 Nucleoporin p62 antibody [2A11].
Left : Nup62 protein focused on nuclear surface
Right : Nup62 protein focused on nuclear rim
Dilution : 1:400
Fixation : 4% PFA
Permeabilization : 0.5% Triton X-100

Anti-Nucleoporin p62 antibody [2A11] used in Western Blot (WB). GTX00696

GTX00696 WB Image

WB analysis of HeLa nuclear membrane extract using GTX00696 Nucleoporin p62 antibody [2A11].
Dilution : 1:500

REFERENCE

Clone Reference

Maeshima K et al. J Cell Sci. 2006; Cell-cycle-dependent dynamics of nuclear pores: pore-free islands and lamins.
Fukuhara T et al. Hybridoma (Larchmt). 2006; 25 (2) Functional analysis of nuclear pore complex protein Nup62/p62 using monoclonal antibodies.
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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399