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OAT antibody [HL2087]

Recombinant antibody Orthogonal Overexpression
Anti-OAT antibody [HL2087] used in Western Blot (WB). GTX637995
Anti-OAT antibody [HL2087] used in Western Blot (WB). GTX637995
Anti-OAT antibody [HL2087] used in IHC (Paraffin sections) (IHC-P). GTX637995
Anti-OAT antibody [HL2087] used in IHC (Paraffin sections) (IHC-P). GTX637995
Anti-OAT antibody [HL2087] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637995

Cat. No. GTX637995

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2087

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all OAT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human OAT

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ornithine aminotransferase , GACR , HOGA , OATASE , OKT

Cellular Localization

Mitochondrion matrix

Background

This gene encodes the mitochondrial enzyme ornithine aminotransferase, which is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. Mutations that result in a deficiency of this enzyme cause the autosomal recessive eye disease Gyrate Atrophy. Alternatively spliced transcript variants encoding different isoforms have been described. Related pseudogenes have been defined on the X chromosome. [provided by RefSeq, Jan 2010]

Database

Research Area

DATA IMAGES

Anti-OAT antibody [HL2087] used in Western Blot (WB). GTX637995

GTX637995 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with OAT antibody [HL2087] (GTX637995) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-OAT antibody [HL2087] used in Western Blot (WB). GTX637995

GTX637995 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with OAT antibody [HL2087] (GTX637995) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-OAT antibody [HL2087] used in IHC (Paraffin sections) (IHC-P). GTX637995

GTX637995 IHC-P Image

OAT antibody [HL2087] detects OAT protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse adrenal gland.
OAT stained by OAT antibody [HL2087] (GTX637995) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-OAT antibody [HL2087] used in IHC (Paraffin sections) (IHC-P). GTX637995

GTX637995 IHC-P Image

OAT antibody [HL2087] detects OAT protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat stomach.
OAT stained by OAT antibody [HL2087] (GTX637995) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-OAT antibody [HL2087] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637995

GTX637995 ICC/IF Image

OAT antibody [HL2087] detects OAT protein at mitochondria by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: OAT stained by OAT antibody [HL2087] (GTX637995) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations