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ORAI3 antibody

KO/KD
Anti-ORAI3 antibody used in IHC (Paraffin sections) (IHC-P). GTX85677
Anti-ORAI3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85677
Anti-ORAI3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85677
Anti-ORAI3 antibody used in Western Blot (WB). GTX85677
Anti-ORAI3 antibody used in Western Blot (WB). GTX85677

Cat. No. GTX85677

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
50 μg ($539)
See all ORAI3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2 - 4 μg/ml
ICC/IF 5 μg/ml
IHC-P 3 μg/ml
ELISA Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Product Note

ORAI3 antibody is predicted to have no cross-reactivity to ORAI1 or ORAI2.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A 19 amino acid synthetic peptide near the C-terminus of human ORAI3. The immunogen is located within amino acids 200 - 250 of ORAI3.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_10733571

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ORAI calcium release-activated calcium modulator 3 , ORAI calcium releaseactivated calcium modulator 3 , ORAI3 , TMEM142C

Cellular Localization

Membrane

Database

Research Area

DATA IMAGES

Anti-ORAI3 antibody used in IHC (Paraffin sections) (IHC-P). GTX85677

GTX85677 IHC-P Image

IHC-P analysis of human placenta tissue using GTX85677 ORAI3 antibody.
Antigen retrieval : Heat-induced antigen retrieval
Dilution : 3 μg/ml

Anti-ORAI3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85677

GTX85677 ICC/IF Image

ICC/IF analysis of A20 cells using GTX85677 ORAI3 antibody.
Dilution : 5μg/ml

Anti-ORAI3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85677

GTX85677 ICC/IF Image

ICC/IF analysis of A20 cells using GTX85677 ORAI3 antibody.
Dilution : 10μg/ml

Anti-ORAI3 antibody used in Western Blot (WB). GTX85677

GTX85677 WB Image

WB analysis of A20 cell lysate using GTX85677 ORAI3 antibody.
Dilution : 2 μg/mL (Lane A) and 4 μg/mL (Lane B)

Anti-ORAI3 antibody used in Western Blot (WB). GTX85677

GTX85677 WB Image

KO/KD

The data was published in the journal Oncotarget in 2018. PMID: 30034631

REFERENCE

Application Reference

Bhattacharya A et al. Oncotarget 2018; 9 (50) : 29468-29483 The calcium channel proteins ORAI3 and STIM1 mediate TGF-帣 induced Snai1 expression.
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 539