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OSBP antibody [N2C1], Internal

Orthogonal
Anti-OSBP antibody [N2C1], Internal used in Western Blot (WB). GTX114602
Anti-OSBP antibody [N2C1], Internal used in Western Blot (WB). GTX114602
Anti-OSBP antibody [N2C1], Internal used in Western Blot (WB). GTX114602
Anti-OSBP antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX114602
Anti-OSBP antibody [N2C1], Internal used in Western Blot (WB). GTX114602

Cat. No. GTX114602

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all OSBP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

Positive Control

Raji , Mouse brain

Predict Reactivity

Rat, Rabbit, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.76 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human OSBP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620134

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

oxysterol binding protein , OSBP1

Cellular Localization

Cytoplasm , Golgi apparatus membrane; Peripheral membrane protein

Background

Oxysterol binding protein is an intracellular protein that is believed to transport sterols from lysosomes to the nucleus where the sterol down-regulates the genes for the LDL receptor, HMG-CoA reductase, and HMG synthetase [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-OSBP antibody [N2C1], Internal used in Western Blot (WB). GTX114602

GTX114602 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
7.5% SDS PAGE
GTX114602 diluted at 1:1000

Anti-OSBP antibody [N2C1], Internal used in Western Blot (WB). GTX114602

GTX114602 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with OSBP antibody [N2C1], Internal (GTX114602) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-OSBP antibody [N2C1], Internal used in Western Blot (WB). GTX114602

GTX114602 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX114602 diluted at 1:1000

Anti-OSBP antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX114602

GTX114602 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H441 xenograft, using OSBP(GTX114602) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-OSBP antibody [N2C1], Internal used in Western Blot (WB). GTX114602

GTX114602 WB Image

Orthogonal

The data was published in the journal Int J Endocrinol in 2018.PMID: 29686703

REFERENCE

Application Reference

Qiong Deng et al. Int J Endocrinol 2018; 2018 : 4537214 Vesicle-Associated Membrane Protein-Associated Protein A Is Involved in Androgen Receptor Trafficking in Mouse Sertoli Cells.
Application : WB
Reactivity : Mouse
Deng Q et al. Hindawi 2018; (Epub) Vesicle-associated membrane protein-associated protein A is involved in androgen receptor trafficking in mouse Sertoli cells.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169