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Oct4 antibody [9B7]

Overexpression
Anti-Oct4 antibody [9B7] used in Western Blot (WB). GTX83850
Anti-Oct4 antibody [9B7] used in IHC (Paraffin sections) (IHC-P). GTX83850
Anti-Oct4 antibody [9B7] used in Western Blot (WB). GTX83850
Anti-Oct4 antibody [9B7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83850
Anti-Oct4 antibody [9B7] used in IHC (Paraffin sections) (IHC-P). GTX83850
Anti-Oct4 antibody [9B7] used in IHC (Paraffin sections) (IHC-P). GTX83850

Cat. No. GTX83850

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 9B7

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Dog
Package
100 μl ($519)
See all Oct4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000
ICC/IF 1:100
IHC-P 1:50
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human POU5F1 (NP_002692) produced in E.coli.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

POU class 5 homeobox 1 , OCT3 , OCT4 , OTF-3 , OTF3 , OTF4 , Oct-3 , Oct-4

Cellular Localization

Cytoplasm,Nucleus

Background

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

Database

Research Area

DATA IMAGES

Anti-Oct4 antibody [9B7] used in Western Blot (WB). GTX83850

GTX83850 WB Image

Overexpression

WB analysis of HEK293T cells transfected with Oct4 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83850 Oct4 antibody [9B7].
Loading : 5 ug per lane

Anti-Oct4 antibody [9B7] used in IHC (Paraffin sections) (IHC-P). GTX83850

GTX83850 IHC-P Image

IHC-P analysis of human ovary tissue using GTX83850 Oct4 antibody [9B7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Oct4 antibody [9B7] used in Western Blot (WB). GTX83850

GTX83850 WB Image

WB analysis of MDCK cell lysate using GTX83850 Oct4 antibody [9B7].
Loading : 10 ug per lane
Dilution : 1:200

Anti-Oct4 antibody [9B7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83850

GTX83850 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with 43377 plasmid using GTX83850 Oct4 antibody [9B7].

Anti-Oct4 antibody [9B7] used in IHC (Paraffin sections) (IHC-P). GTX83850

GTX83850 IHC-P Image

IHC-P analysis of human endometrium tissue using GTX83850 Oct4 antibody [9B7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Oct4 antibody [9B7] used in IHC (Paraffin sections) (IHC-P). GTX83850

GTX83850 IHC-P Image

IHC-P analysis of human lung tissue using GTX83850 Oct4 antibody [9B7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519