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Orai1 antibody

Anti-Orai1 antibody used in Flow cytometry (FACS). GTX54826
Anti-Orai1 antibody used in Western Blot (WB). GTX54826

Cat. No. GTX54826

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS, IHC

Reactivity

 Human
Package
50 μl ($639)
See all Orai1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Product Note

We do not recommend use of this product for Mouse,Rat samples.

Predict Reactivity

Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide (C)KKQPGQPRPTSK, corresponding to amino acid residues 203-214 (2nd  extracellular loop) of human Orai1 (Accession  : Q96D31).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ORAI calcium release-activated calcium modulator 1 , CRACM1 , IMD9 , ORAT1 , TAM2 , TMEM142A

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a membrane calcium channel subunit that is activated by the calcium sensor STIM1 when calcium stores are depleted. This type of channel is the primary way for calcium influx into T-cells. Defects in this gene are a cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 1 (IDTICED1). [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-Orai1 antibody used in Flow cytometry (FACS). GTX54826

GTX54826 FACS Image

FACS analysis of HL-60 (A)  and Jurkat (B) cells using GTX54826 Orai1 antibody.
Black : Unstained cell
Green : Cell staining with primary antibody
Antibody amount : 5-10 μg antibody / 0.5-1 x 10⁶  cells

Anti-Orai1 antibody used in Western Blot (WB). GTX54826

GTX54826 WB Image

WB analysis of Jurkat (lanes 1 and 2) and HL-60 (lanes 3 and 4) cell lysates using GTX54826 Orai1 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 639