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P-Cadherin antibody [6A9]

Anti-P-Cadherin antibody [6A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX19350
Anti-P-Cadherin antibody [6A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX19350
Anti-P-Cadherin antibody [6A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX19350
Anti-P-Cadherin antibody [6A9] used in Western Blot (WB). GTX19350

Cat. No. GTX19350

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6A9

Isotype

 IgG1

Application

 WB, ICC/IF, IP, Neutralizing/Inhibition

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all P-Cadherin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100-1:1000
ICC/IF 10 μg/ml
IP Assay dependent
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

Calculated MW

91 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A431 trypsinized membranes.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cadherin 3 , CDHP , HJMD , PCAD

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Background

This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. This gene is located in a gene cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene are associated with hypotrichosis with juvenile macular dystrophy and ectodermal dysplasia, ectrodactyly, and macular dystrophy syndrome (EEMS). [provided by RefSeq, Nov 2015]

Database

Research Area

DATA IMAGES

Anti-P-Cadherin antibody [6A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX19350

GTX19350 ICC/IF Image

ICC/IF analysis of BEAS-2B Cells using GTX19350 P-Cadherin antibody [6A9]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

Anti-P-Cadherin antibody [6A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX19350

GTX19350 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX19350 P-Cadherin antibody [6A9]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

Anti-P-Cadherin antibody [6A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX19350

GTX19350 ICC/IF Image

ICC/IF analysis of A2058 Cells using GTX19350 P-Cadherin antibody [6A9]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

Anti-P-Cadherin antibody [6A9] used in Western Blot (WB). GTX19350

GTX19350 WB Image

WB analysis of 25 ug of 293T (lane 1), A431 (lane 2) and mouse brain (lane 3) using GTX19350 P-Cadherin antibody [6A9].
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399