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P Glycoprotein antibody [UIC2]

Cat. No. GTX42220

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 UIC2

Isotype

 IgG2a

Application

 ICC/IF, IHC-P, IHC-Fr, FACS, IP

Reactivity

 Human
Package
100 μg ($399)
See all P Glycoprotein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS 1/50-1/200
IP Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
Not tested in other applications.

Calculated MW

141 kDa. ( Note )

Product Note

This antibody recognizes an extracellular conformational epitope of CD243, also known as MDR1 (multi-drug resistance protein 1) and Pgp (P-glycoprotein).

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Mouse Balb/c 3T3 fibroblasts transfected with human P Glycoprotein cDNA.

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_11173104

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATP binding cassette subfamily B member 1 , ABC20 , CD243 , CLCS , GP170 , MDR1 , P-GP , PGY1

Cellular Localization

Cell membrane

Background

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. Mutations in this gene are associated with colchicine resistance and Inflammatory bowel disease 13. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Feb 2017]

Database

Research Area

REFERENCE

Application Reference

Ammar M et al. J Clin Lab Anal 2020; 34 (9) : e23374 Overexpression of P-glycoprotein and resistance to Imatinib in chronic myeloid leukemia patients.
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399